UZH-Logo

Maintenance Infos

Lag effect of microclimatic conditions on DNA integrity of frozen-thawed bovine sperm


Malama, E; Kiossis, E; Theodosiou, T; Boscos, C; Bollwein, H (2012). Lag effect of microclimatic conditions on DNA integrity of frozen-thawed bovine sperm. Animal Reproduction Science, 136(1-2):33-41.

Abstract

The objective of this study was to investigate the effect of microclimatic conditions on DNA integrity of bovine sperm. DNA fragmentation of frozen-thawed sperm was analyzed in monthly semen samples of nine AI bulls ejaculating in weekly routine for one year. The extent of DNA fragmentation was determined using the Sperm Chromatin Structure Assay (SCSA™) and quantified by the DNA fragmentation index (DFI) and the percentage of sperm showing high DFI values (%DFI) immediately (0h) and 3h (3h) after thawing. Microclimatic factors (ambient air temperature, relative humidity, dew point) were recorded in hourly intervals throughout the year. Their mean values were calculated for seven different time periods (up to day 56) preceding ejaculation (day 0). DFI (P<0.01) and its relative change between 0h and 3h (P<0.05) were related to the set of microclimatic parameters recorded the last 11 days and from day 49 to 43 prior to ejaculation, respectively. A significant relationship was detected between %DFI and microclimatic parameters of days 35-29 preceding ejaculation (P<0.05), while the degree of change of %DFI from 0h to 3h was related to microclimatic parameters recorded from day 28 to 22 before ejaculation (P<0.05). Dew point and relative humidity appeared to be the strongest and weakest predictors of DNA integrity, respectively. In conclusion, the results of the present study showed a lag effect of microclimate on DNA integrity of frozen-thawed bovine sperm. However, microclimatic parameters of a single time period before ejaculation could not be identified as the source of variation of different SCSA parameters.

The objective of this study was to investigate the effect of microclimatic conditions on DNA integrity of bovine sperm. DNA fragmentation of frozen-thawed sperm was analyzed in monthly semen samples of nine AI bulls ejaculating in weekly routine for one year. The extent of DNA fragmentation was determined using the Sperm Chromatin Structure Assay (SCSA™) and quantified by the DNA fragmentation index (DFI) and the percentage of sperm showing high DFI values (%DFI) immediately (0h) and 3h (3h) after thawing. Microclimatic factors (ambient air temperature, relative humidity, dew point) were recorded in hourly intervals throughout the year. Their mean values were calculated for seven different time periods (up to day 56) preceding ejaculation (day 0). DFI (P<0.01) and its relative change between 0h and 3h (P<0.05) were related to the set of microclimatic parameters recorded the last 11 days and from day 49 to 43 prior to ejaculation, respectively. A significant relationship was detected between %DFI and microclimatic parameters of days 35-29 preceding ejaculation (P<0.05), while the degree of change of %DFI from 0h to 3h was related to microclimatic parameters recorded from day 28 to 22 before ejaculation (P<0.05). Dew point and relative humidity appeared to be the strongest and weakest predictors of DNA integrity, respectively. In conclusion, the results of the present study showed a lag effect of microclimate on DNA integrity of frozen-thawed bovine sperm. However, microclimatic parameters of a single time period before ejaculation could not be identified as the source of variation of different SCSA parameters.

Citations

3 citations in Web of Science®
3 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 30 Jan 2013
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:2012
Deposited On:30 Jan 2013 10:34
Last Modified:05 Apr 2016 16:27
Publisher:Elsevier
ISSN:0378-4320
Publisher DOI:https://doi.org/10.1016/j.anireprosci.2012.10.017
PubMed ID:23182467
Permanent URL: https://doi.org/10.5167/uzh-72621

Download

[img]
Content: Published Version
Language: English
Filetype: PDF - Registered users only
Size: 540kB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations