Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-742
Hulf, T; Bellosta, P; Furrer, M; Steiger, D; Svensson, D; Barbour, A D; Gallant, P (2005). Whole-genome analysis reveals a strong positional bias of conserved dMyc-dependent E-boxes. Molecular and Cellular Biology, 25(9):3401-3410.
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Myc is a transcription factor with diverse biological effects ranging from the control of cellular proliferation and growth to the induction of apoptosis. Here we present a comprehensive analysis of the transcriptional targets of the sole Myc ortholog in Drosophila melanogaster, dMyc. We show that the genes that are down-regulated in response to dmyc inhibition are largely identical to those that are up-regulated after dMyc overexpression and that many of them play a role in growth control. The promoter regions of these targets are characterized by the presence of the E-box sequence CACGTG, a known dMyc binding site. Surprisingly, a large subgroup of (functionally related) dMyc targets contains a single E-box located within the first 100 nucleotides after the transcription start site. The relevance of this E-box and its position was confirmed by a mutational analysis of a selected dMyc target and by the observation of its evolutionary conservation in a different Drosophila species, Drosophila pseudoobscura. These observations raise the possibility that a subset of Myc targets share a distinct regulatory mechanism.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Zoology (former)|
|Dewey Decimal Classification:||570 Life sciences; biology
590 Animals (Zoology)
|Date:||1 May 2005|
|Deposited On:||11 Feb 2008 12:17|
|Last Modified:||27 Nov 2013 19:26|
|Publisher:||American Society for Microbiology (ASM)|
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