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Superresolution imaging of amyloid fibrils with binding-activated probes


Ries, Jonas; Udayar, Vinod; Soragni, Alice; Hornemann, Simone; Nilsson, K Peter R; Riek, Roland; Hock, Christoph; Ewers, Helge; Aguzzi, Adriano A; Rajendran, Lawrence (2013). Superresolution imaging of amyloid fibrils with binding-activated probes. ACS Chemical Neuroscience, 4(7):1057-1061.

Abstract

Protein misfolding into amyloid-like aggregates underlies many neurodegenerative diseases. Thus, insights into the structure and function of these amyloids will provide valuable information on the pathological mechanisms involved and aid in the design of improved drugs for treating amyloid-based disorders. However, determining the structure of endogenous amyloids at high resolution has been difficult. Here we employ binding-activated localization microscopy (BALM) to acquire superresolution images of α-synuclein amyloid fibrils with unprecedented optical resolution. We propose that BALM imaging can be extended to study the structure of other amyloids, for differential diagnosis of amyloid-related diseases and for discovery of drugs that perturb amyloid structure for therapy.

Protein misfolding into amyloid-like aggregates underlies many neurodegenerative diseases. Thus, insights into the structure and function of these amyloids will provide valuable information on the pathological mechanisms involved and aid in the design of improved drugs for treating amyloid-based disorders. However, determining the structure of endogenous amyloids at high resolution has been difficult. Here we employ binding-activated localization microscopy (BALM) to acquire superresolution images of α-synuclein amyloid fibrils with unprecedented optical resolution. We propose that BALM imaging can be extended to study the structure of other amyloids, for differential diagnosis of amyloid-related diseases and for discovery of drugs that perturb amyloid structure for therapy.

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16 citations in Web of Science®
19 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
04 Faculty of Medicine > Institute for Regenerative Medicine (IREM)
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2013
Deposited On:02 May 2013 08:10
Last Modified:16 Aug 2016 10:12
Publisher:American Chemical Society
ISSN:1948-7193
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1021/cn400091m
PubMed ID:23594172
Permanent URL: https://doi.org/10.5167/uzh-77890

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