UZH-Logo

Maintenance Infos

Genome-wide expression analysis of ptf1a- and ascl1-deficient mice reveals new markers for distinct dorsal horn interneuron populations contributing to nociceptive reflex plasticity


Wildner, Hendrik; Das Gupta, Rebecca; Bröhl, Dominique; Heppenstall, Paul A; Zeilhofer, Hanns Ulrich; Birchmeier, Carmen (2013). Genome-wide expression analysis of ptf1a- and ascl1-deficient mice reveals new markers for distinct dorsal horn interneuron populations contributing to nociceptive reflex plasticity. Journal of Neuroscience, 33(17):7299-7307.

Abstract

Inhibitory interneurons of the spinal dorsal horn play critical roles in the processing of noxious and innocuous sensory information. They form a family of morphologically and functionally diverse neurons that likely fall into distinct subtypes. Traditional classifications rely mainly on differences in dendritic tree morphology and firing patterns. Although useful, these markers are not comprehensive and cannot be used to drive specific genetic manipulations targeted at defined subsets of neurons. Here, we have used genome-wide expression profiling of spinal dorsal horns of wild-type mice and of two strains of transcription factor-deficient mice (Ptf1a(-/-) and Ascl1/Mash1(-/-) mice) to identify new genetic markers for specific subsets of dorsal horn inhibitory interneurons. Ptf1a(-/-) mice lack all inhibitory interneurons in the dorsal horn, whereas only the late-born inhibitory interneurons are missing in Ascl1(-/-) mice. We found 30 genes that were significantly downregulated in the dorsal horn of Ptf1a(-/-) mice. Twenty-one of those also showed reduced expression in Ascl1(-/-) mice. In situ hybridization analyses of all 30 genes identified four genes with primarily non-overlapping expression patterns in the dorsal horn. Three genes, pDyn coding the neuropeptide dynorphin, Kcnip2 encoding a potassium channel associated protein, and the nuclear receptor encoding gene Rorb, were expressed in Ascl1-dependent subpopulations of the superficial dorsal horn. The fourth gene, Tfap2b, encoding a transcription factor, is expressed mainly in a Ascl1-independent subpopulation of the deep dorsal horn. Functional experiments in isolated spinal cords showed that the Ascl1-dependent inhibitory interneurons are key players of nociceptive reflex plasticity.

Inhibitory interneurons of the spinal dorsal horn play critical roles in the processing of noxious and innocuous sensory information. They form a family of morphologically and functionally diverse neurons that likely fall into distinct subtypes. Traditional classifications rely mainly on differences in dendritic tree morphology and firing patterns. Although useful, these markers are not comprehensive and cannot be used to drive specific genetic manipulations targeted at defined subsets of neurons. Here, we have used genome-wide expression profiling of spinal dorsal horns of wild-type mice and of two strains of transcription factor-deficient mice (Ptf1a(-/-) and Ascl1/Mash1(-/-) mice) to identify new genetic markers for specific subsets of dorsal horn inhibitory interneurons. Ptf1a(-/-) mice lack all inhibitory interneurons in the dorsal horn, whereas only the late-born inhibitory interneurons are missing in Ascl1(-/-) mice. We found 30 genes that were significantly downregulated in the dorsal horn of Ptf1a(-/-) mice. Twenty-one of those also showed reduced expression in Ascl1(-/-) mice. In situ hybridization analyses of all 30 genes identified four genes with primarily non-overlapping expression patterns in the dorsal horn. Three genes, pDyn coding the neuropeptide dynorphin, Kcnip2 encoding a potassium channel associated protein, and the nuclear receptor encoding gene Rorb, were expressed in Ascl1-dependent subpopulations of the superficial dorsal horn. The fourth gene, Tfap2b, encoding a transcription factor, is expressed mainly in a Ascl1-independent subpopulation of the deep dorsal horn. Functional experiments in isolated spinal cords showed that the Ascl1-dependent inhibitory interneurons are key players of nociceptive reflex plasticity.

Citations

11 citations in Web of Science®
12 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 20 Aug 2013
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2013
Deposited On:20 Aug 2013 06:49
Last Modified:05 Apr 2016 16:47
Publisher:Society for Neuroscience
ISSN:0270-6474
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1523/JNEUROSCI.0491-13.2013
PubMed ID:23616538
Permanent URL: https://doi.org/10.5167/uzh-78217

Download

[img]
Content: Published Version
Language: English
Filetype: PDF - Registered users only
Size: 2MB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations