Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-789
Hottiger, M O; Gramatikoff, K; Georgiev, O; Chaponnier, C; Schaffner, W; Hübscher, U (1995). The large subunit of HIV-1 reverse transcriptase interacts with beta-actin. Nucleic Acids Research, 23(5):736-741.
HIV-1 reverse transcriptase is a dimeric enzyme mainly involved in the replication of the viral genome. A filamentous phage cDNA expression library from human lymphocytes was used to select cellular proteins interacting with HIV-1 reverse transcriptase Affinity selections using the bacterially expressed monomeric large subunit of reverse transcriptase (p66) yielded host beta-actin. This clone was expressed as glutathione-S-transferase fusion protein which was identified by using a specific antibody against beta-actin. Furthermore we show that also the eukaryotic beta-actin binds to either the large subunit of reverse transcriptase or to the Pol precursor polyprotein in vitro. The reverse transcriptase/beta-actin interaction might be important for the secretion of HIV-1 virions.
|Item Type:||Journal Article, refereed|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology|
|DDC:||570 Life sciences; biology|
|Date:||11 March 1995|
|Deposited On:||11 Feb 2008 13:18|
|Last Modified:||23 Nov 2012 16:59|
|Publisher:||Oxford University Press|
|WoS Citation Count:||44|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page