Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-7968
Bolinger, B; Krebs, P; Tian, Y; Engeler, D; Scandella, E; Miller, S; Palmer, D C; Restifo, N P; Clavien, P A; Ludewig, B (2008). Immunologic ignorance of vascular endothelial cells expressing minor histocompatibility antigen. Blood, 111(9):4588-4595.
View at publisher
Endothelial cells (ECs) presenting minor histocompatibility antigen (mhAg) are major target cells for alloreactive effector CD8(+) T cells during chronic transplant rejection and graft-versus-host disease (GVHD). The contribution of ECs to T-cell activation, however, is still a controversial issue. In this study, we have assessed the antigen-presenting capacity of ECs in vivo using a transgenic mouse model with beta-galactosidase (beta-gal) expression confined to the vascular endothelium (Tie2-LacZ mice). In a GVHD-like setting with adoptive transfer of beta-gal-specific T-cell receptor-transgenic T cells, beta-gal expression by ECs was not sufficient to either activate or tolerize CD8(+) T cells. Likewise, transplantation of fully vascularized heart or liver grafts from Tie2-LacZ mice into nontransgenic recipients did not suffice to activate beta-gal-specific CD8(+) T cells, indicating that CD8(+) T-cell responses against mhAg cannot be initiated by ECs. Moreover, we could show that spontaneous activation of beta-gal-specific CD8(+) T cells in Tie2-LacZ mice was exclusively dependent on CD11c(+) dendritic cells (DCs), demonstrating that mhAgs presented by ECs remain immunologically ignored unless presentation by DCs is granted.
175 downloads since deposited on 11 Dec 2008
64 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Visceral and Transplantation Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
|Dewey Decimal Classification:||610 Medicine & health|
|Deposited On:||11 Dec 2008 14:38|
|Last Modified:||27 Nov 2013 21:50|
|Publisher:||American Society of Hematology|
|Additional Information:||This research was originally published in Blood, 2008; 111(9). Copyright by the American Society of Hematology.|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page