Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-831

Hoffmann, J S; Pillaire, M J; Maga, G; Podust, V N; Hübscher, U; Villani, G (1995). DNA polymerase beta bypasses in vitro a single d(GpG)-cisplatin adduct placed on codon 13 of the HRAS gene. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 92(12):5356-5360.

[img]
Preview
PDF
1MB

View at publisher

Abstract

We have examined the capacity of calf thymus DNA polymerases alpha, beta, delta, and epsilon to perform in vitro translesion synthesis on a substrate containing a single d(GpG)-cisplatin adduct placed on codon 13 of the human HRAS gene. We found that DNA synthesis catalyzed by DNA polymerases alpha, delta, and epsilon was blocked at the base preceding the lesion. Addition of proliferating cell nuclear antigen to DNA polymerase delta and replication protein A to DNA polymerase alpha did not restore their capacity to elongate past the adduct. On the other hand, DNA polymerase beta efficiently bypassed the cisplatin adduct. Furthermore, we observed that DNA polymerase beta was the only polymerase capable of primer extension of a 3'-OH located opposite the base preceding the lesion. Likewise, DNA polymerase beta was able to elongate the arrested replication products of the other three DNA polymerases, thus showing its capacity to successfully compete with polymerases alpha, delta, and epsilon in the stalled replication complex. Our data suggest (i) a possible mechanism enabling DNA polymerase beta to bypass a d(GpG)-cisplatin adduct in vitro and (ii) a role for this enzyme in processing DNA damage in vivo.

Citations

114 citations in Web of Science®
112 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

13 downloads since deposited on 11 Feb 2008
6 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology
DDC:570 Life sciences; biology
Language:English
Date:6 June 1995
Deposited On:11 Feb 2008 12:18
Last Modified:27 Nov 2013 17:10
Publisher:National Academy of Sciences
ISSN:0027-8424
Publisher DOI:10.1073/pnas.92.12.5356
Related URLs:http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=41693&blobtype=pdf
PubMed ID:7777511

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page