Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Henneke, G; Raffin, J P; Ferrari, E; Jónsson, Z O; Dietrich, J; Hübscher, U (2000). The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta. Biochemical and Biophysical Research Communications (BBRC), 276(2):600-6.

Full text not available from this repository.

View at publisher


We have cloned the gene encoding proliferating cell nuclear antigen (PCNA) from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M(r) of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNA were required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta. Our data suggest that PCNA has been functionally conserved not only within eukaryotes but also from hyperthermophilic euryarchaeotes to mammals.


15 citations in Web of Science®
18 citations in Scopus®
Google Scholar™


Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology
Dewey Decimal Classification:570 Life sciences; biology
Date:24 September 2000
Deposited On:11 Feb 2008 12:18
Last Modified:05 Apr 2016 12:16
Publisher DOI:10.1006/bbrc.2000.3481
Related URLs:http://www.sciencedirect.com/science/journal/0006291X
PubMed ID:11027519

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page