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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-848

Stagljar, I; Hübscher, U; Barberis, A (1999). Activation of DNA replication in yeast by recruitment of the RNA polymerase II transcription complex. Biological Chemistry, 380(5):525-30.



Activators of transcription are known to also play an important and direct role in activating DNA replication. However, the mechanism whereby they stimulate replication has remained elusive. One model suggests that, in the context of replication origins, transcriptional activators work by interacting with replication factors. We show that a defined, single interaction between a DNA-bound derivative of the activator Gal4 and Gal11P, a mutant form of the RNA polymerase II holoenzyme component Gal11, suffices for stimulating DNA replication as it does for transcription. Moreover, recruitment of TBP, which can activate transcription from a gene promoter, also stimulates DNA replication from an origin site. These results strongly argue that transcriptional activators may not necessarily need to contact DNA replication factors directly, but can stimulate replication by recruiting the RNA polymerase II transcription complex to DNA.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology
DDC:570 Life sciences; biology
Date:1 May 1999
Deposited On:11 Feb 2008 12:18
Last Modified:27 Nov 2013 19:26
Publisher:De Gruyter
Publisher DOI:10.1515/BC.1999.067
PubMed ID:10384958
Citations:Web of Science®. Times Cited: 20
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Scopus®. Citation Count: 19

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