Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Maintenance: Tuesday, 5.7.2016, 07:00-08:00

Maintenance work on ZORA and JDB on Tuesday, 5th July, 07h00-08h00. During this time there will be a brief unavailability for about 1 hour. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-8718

Schegg, B; Hülsmeier, A J; Rutschmann, C; Maag, C; Hennet, T (2009). Core glycosylation of collagen is initiated by two {beta}(1-O)galactosyltransferases. Molecular and Cellular Biology, 29(4):943-952.

Accepted Version
View at publisher


Collagen is a trimer of three left-handed alpha-chains representing repeats of the motif Gly-X-Y, whereas (hydroxy)proline and (hydroxy)lysine residues are often found at positions X and Y. Selected hydroxylysine are further modified by the addition of galactose and glucose-galactose units. Collagen glycosylation takes place in the endoplasmic reticulum before triple helix formation and is mediated by beta(1-O)galactosyl- and alpha(1-2)glucosyltransferase enzymes. We have identified two collagen galactosyltransferases using affinity chromatography and tandem-MS protein sequencing. The two collagen beta(1-O)galactosyltransferases corresponded to the GLT25D1 and GLT25D2 proteins. Recombinant GLT25D1 and GLT25D2 enzymes showed a strong galactosyltransferase activity towards various types of collagen and towards the serum mannose-binding lectin MBL, which contains a collagen domain. Amino acid analysis of the products of GLT25D1 and GLT25D2 reactions confirmed the transfer of galactose to hydroxylysine residues. The GLT25D1 gene is constitutively expressed in human tissues, whereas the GLT25D2 gene is only expressed at low levels in the nervous system. The GLT25D1 and GLT25D2 enzymes are similar to CEECAM1, to which we could not attribute any collagen galactosyltransferase activity. The GLT25D1 and GLT25D2 genes now allow addressing the biological significance of collagen glycosylation and the importance of this post-translational modification in the etiology of connective tissue disorders.


40 citations in Web of Science®
39 citations in Scopus®
Google Scholar™



84 downloads since deposited on 19 Dec 2008
15 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Deposited On:19 Dec 2008 08:42
Last Modified:05 Apr 2016 12:44
Publisher:American Society for Microbiology
Additional Information:Copyright: American Society for Microbiology.
Publisher DOI:10.1128/MCB.02085-07
PubMed ID:19075007

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page