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Static biofilm removal around ultrasonic tips in vitro


Thurnheer, Thomas; Rohrer, Elodie; Belibasakis, Georgios N; Attin, Thomas; Schmidlin, Patrick R (2014). Static biofilm removal around ultrasonic tips in vitro. Clinical Oral Investigations, 18(7):1779-1784.

Abstract

OBJECTIVES: This study aims to investigate the biofilm removal capacity of two ultrasonic tips under standardized conditions using a multi-species biofilm model. METHODS: Six-species biofilms were grown on hydroxyapatite discs for 64.5 h and were treated for 15 s with a standardized load of 40 g with a piezoelectric or magnetostrictive device. Tips were applied either with the tip end or with the side facing downwards. Detached bacteria were determined in the supernatant and colony-forming units (CFUs) counted after 72 h of incubation. Untreated specimens served as controls. Moreover, the biofilms remaining on the hydroxyapatite surface after treatment were stained using the Live/Dead stain, and the pattern of their detachment was assessed by confocal laser scanning microscopy (CLSM). RESULTS: As compared to the untreated control, it was found that only a side application of the magnetostrictive device was able to remove efficiently the biofilm. In contrast, its tip application as well as both applications of the piezoelectric device removed significantly less bacteria from the biofilm structure. These findings were corroborated by CLSM observation. CONCLUSION: Both ultrasonic tips under investigations led to bacterial detachment, but the action mode as well as the tip configuration and adaptation appeared to be influenced by the biofilm removal effectiveness. CLINICAL RELEVANCE: Biofilm removal remains a main goal of ultrasonic debridement. This should be reflected in respective laboratory investigations. The presented combination of methods applied on a multi-species biofilm model in vitro allows the evaluation of the effectiveness of different ultrasonic scaler applications.

OBJECTIVES: This study aims to investigate the biofilm removal capacity of two ultrasonic tips under standardized conditions using a multi-species biofilm model. METHODS: Six-species biofilms were grown on hydroxyapatite discs for 64.5 h and were treated for 15 s with a standardized load of 40 g with a piezoelectric or magnetostrictive device. Tips were applied either with the tip end or with the side facing downwards. Detached bacteria were determined in the supernatant and colony-forming units (CFUs) counted after 72 h of incubation. Untreated specimens served as controls. Moreover, the biofilms remaining on the hydroxyapatite surface after treatment were stained using the Live/Dead stain, and the pattern of their detachment was assessed by confocal laser scanning microscopy (CLSM). RESULTS: As compared to the untreated control, it was found that only a side application of the magnetostrictive device was able to remove efficiently the biofilm. In contrast, its tip application as well as both applications of the piezoelectric device removed significantly less bacteria from the biofilm structure. These findings were corroborated by CLSM observation. CONCLUSION: Both ultrasonic tips under investigations led to bacterial detachment, but the action mode as well as the tip configuration and adaptation appeared to be influenced by the biofilm removal effectiveness. CLINICAL RELEVANCE: Biofilm removal remains a main goal of ultrasonic debridement. This should be reflected in respective laboratory investigations. The presented combination of methods applied on a multi-species biofilm model in vitro allows the evaluation of the effectiveness of different ultrasonic scaler applications.

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4 citations in Web of Science®
3 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:September 2014
Deposited On:20 Dec 2013 10:09
Last Modified:05 Apr 2016 17:18
Publisher:Springer
ISSN:1432-6981
Additional Information:The final publication is available at link.springer.com
Publisher DOI:https://doi.org/10.1007/s00784-013-1157-2
PubMed ID:24317957
Permanent URL: https://doi.org/10.5167/uzh-87186

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