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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-8752

Grieshaber, D; de Lange, V; Hirt, T; Lu, Z; Vörös, J (2008). Vesicles for signal amplification in a biosensor for the detection of low antigen concentrations. Sensors, 8(12):7894-7903.

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Abstract

The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
DDC:170 Ethics
610 Medicine & health
Language:English
Date:2008
Deposited On:13 Jan 2009 08:22
Last Modified:18 Jul 2014 18:04
Publisher:Molecular Diversity Preservation International (MDPI)
ISSN:1424-8220
Publisher DOI:10.3390/s8127894
Citations:Web of Science®. Times Cited: 8
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Scopus®. Citation Count: 8

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