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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-8762

Schellenberg, S; Grenacher, B; Kaufmann, K; Reusch, C E; Glaus, T M (2008). Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog. Veterinary Journal, 178(1):85-90.

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Abstract

Immunoassays for the measurement of concentrations of the cardiovascular peptides pro-atrial natriuretic peptide (proANP), brain natriuretic peptide (BNPPen and BNPPhoe), endothelin-1 (ET-1Bio, ET-1IBL and ET-1Phoe) and big endothelin-1 (Big-ETBio and Big-ETIBL) were validated in canine serum by determination of intra-assay variability and dilutional parallelism. Commercial kits that showed good results were further validated by determination of intra- and inter-assay variability, dilutional parallelism and spiking recovery. Assays for proANP, BNPPhoe, ET-1IBL and Big-ETIBL showed acceptable results in the preliminary validation and were fully validated. The intra- and inter-assay variability was acceptable for all four assays, linearity was demonstrated and recovery rates were acceptable. The performances of the different immunoassays varied considerably, underscoring the importance of validation. Of the assays studied, proANP, BNP(Phoe), ET-1IBL and Big-ETIBL produced precise, reproducible and accurate results and can be recommended for clinical application.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals > Clinic for Small Animal Internal Medicine
DDC:570 Life sciences; biology
630 Agriculture
Language:English
Date:October 2008
Deposited On:05 Jan 2009 15:01
Last Modified:27 Nov 2013 22:50
Publisher:Elsevier
ISSN:1090-0233
Publisher DOI:10.1016/j.tvjl.2007.07.002
PubMed ID:17716935
Citations:Web of Science®. Times Cited: 13
Google Scholar™
Scopus®. Citation Count: 12

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