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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-9279

Sigurdson, C J; Nilsson, K P R; Hornemann, S; Heikenwalder, M; Manco, G; Schwarz, P; Ott, D; Rülicke, T; Liberski, P P; Julius, C; Falsig, J; Stitz, L; Wüthrich, K; Aguzzi, A (2009). De novo generation of a transmissible spongiform encephalopathy by mouse transgenesis. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 106(1):304-309.

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Most transmissible spongiform encephalopathies arise either spontaneously or by infection. Mutations of PRNP, which encodes the prion protein, PrP, segregate with phenotypically similar diseases. Here we report that moderate overexpression in transgenic mice of mPrP(170N,174T), a mouse PrP with two point mutations that subtly affect the structure of its globular domain, causes a fully penetrant lethal spongiform encephalopathy with cerebral PrP plaques. This genetic disease was reproduced with 100% attack rate by intracerebral inoculation of brain homogenate to tga20 mice overexpressing WT PrP, and from the latter to WT mice, but not to PrP-deficient mice. Upon successive transmissions, the incubation periods decreased and PrP became more protease-resistant, indicating the presence of a strain barrier that was gradually overcome by repeated passaging. This shows that expression of a subtly altered prion protein, with known 3D structure, efficiently generates a prion disease.


107 citations in Web of Science®
125 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Date:6 January 2009
Deposited On:08 Jan 2009 13:51
Last Modified:05 Apr 2016 12:46
Publisher:National Academy of Sciences
Additional Information:Copyright: National Academy of Sciences USA
Publisher DOI:10.1073/pnas.0810680105
PubMed ID:19073920

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