Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-9281
Heikenwalder, M; Kurrer, M O; Margalith, I; Kranich, J; Zeller, N; Haybaeck, J; Polymenidou, M; Matter, M; Bremer, J; Jackson, W S; Lindquist, S; Sigurdson, C J; Aguzzi, A (2008). Lymphotoxin-dependent prion replication in inflammatory stromal cells of granulomas. Immunity, 29(6):998-1008.
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Prior to invading the nervous system, prions frequently colonize lymphoid organs and sites of inflammatory lymphoneogenesis, where they colocalize with Mfge8(+) follicular dendritic cells (FDCs). Here, we report that soft-tissue granulomas, a frequent feature of chronic inflammation, expressed the cellular prion protein (PrP(C), encoded by Prnp) and the lymphotoxin receptor (LTbetaR), even though they lacked FDCs and did not display lymphoneogenesis. After intraperitoneal prion inoculation, granulomas of Prnp(+/+) mice, but not Prnp(-/-) granulomas or unaffected Prnp(+/+) skin, accumulated prion infectivity and disease-associated prion protein. Bone-marrow transfers between Prnp(+/+) and Prnp(-/-) mice and administration of lymphotoxin signaling antagonists indicated that prion replication required radioresistant PrP(C)-expressing cells and LTbetaR signaling. Granulomatous PrP(C) was mainly expressed by stromal LTbetaR(+) mesenchymal cells that were absent from unaffected subcutis. Hence, granulomas can act as clinically silent reservoirs of prion infectivity. Furthermore, lymphotoxin-dependent prion replication can occur in inflammatory stromal cells that are distinct from FDCs.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||21 Jan 2009 22:20|
|Last Modified:||28 Nov 2013 01:43|
|Citations:||Web of Science®. Times cited: 27|
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