Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-945
Kritikou, E A; Milstein, S; Vidalain, P O; Lettre, G; Bogan, E; Doukoumetzidis, K; Gray, P; Chappell, T G; Vidal, M; Hengartner, M O (2006). C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway required for germ cell survival. Genes and Development, 20(16):2279-2292.
During oocyte development in Caenorhabditis elegans, approximately half of all developing germ cells undergo apoptosis. While this process is evolutionarily conserved from worms to humans, the regulators of germ cell death are still largely unknown. In a genetic screen for novel genes involved in germline apoptosis in Caenorhabditis elegans, we identified and cloned gla-3. Loss of gla-3 function results in increased germline apoptosis and reduced brood size due to defective pachytene exit from meiosis I. gla-3 encodes a TIS11-like zinc-finger-containing protein that is expressed in the germline, from the L4 larval stage to adulthood. Biochemical evidence and genetic epistasis analysis revealed that GLA-3 participates in the MAPK signaling cascade and directly interacts with the C. elegans MAPK MPK-1, an essential meiotic regulator. Our results show that GLA-3 is a new component of the MAPK cascade that controls meiotic progression and apoptosis in the C. elegans germline and functions as a negative regulator of the MAPK signaling pathway during vulval development and in muscle cells.
|Item Type:||Journal Article, refereed|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Date:||15 August 2006|
|Deposited On:||11 Feb 2008 12:19|
|Last Modified:||27 Nov 2013 18:45|
|Publisher:||Cold Spring Harbor Laboratory Press|
|Citations:||Web of Science®. Times Cited: 25|
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