Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-947
Degtyareva, N P; Greenwell, P; Hofmann, E R; Hengartner, M O; Zhang, L; Culotti, J G; Petes, T D (2002). Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 99(4):2158-2163.
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Mismatch repair genes are important in maintaining the fidelity of DNA replication. To determine the function of the Caenorhabditis elegans homologue of the MSH2 mismatch repair gene (msh-2), we isolated a strain of C. elegans with an insertion of the transposable element Tc1 within msh-2. Early-passage msh-2 mutants were similar to wild-type worms with regard to lifespan and meiotic chromosome segregation but had slightly reduced fertility. The mutant worms had reduced DNA damage-induced germ-line apoptosis after genotoxic stress. The msh-2 mutants also had elevated levels of microsatellite instability and increased rates of reversion of the dominant unc-58(e665) mutation. In addition, serially passaged cultures of msh-2 worms died out much more quickly than those of wild-type worms. These results demonstrate that msh-2 function in C. elegans is important in regulating both short- and long-term genomic stability.
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|Item Type:||Journal Article, refereed|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|Dewey Decimal Classification:||570 Life sciences; biology|
|Date:||19 February 2002|
|Deposited On:||11 Feb 2008 12:19|
|Last Modified:||28 Nov 2013 01:19|
|Publisher:||National Academy of Sciences|
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