Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-947
Degtyareva, N P; Greenwell, P; Hofmann, E R; Hengartner, M O; Zhang, L; Culotti, J G; Petes, T D (2002). Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 99(4):2158-2163.
View at publisher
Mismatch repair genes are important in maintaining the fidelity of DNA replication. To determine the function of the Caenorhabditis elegans homologue of the MSH2 mismatch repair gene (msh-2), we isolated a strain of C. elegans with an insertion of the transposable element Tc1 within msh-2. Early-passage msh-2 mutants were similar to wild-type worms with regard to lifespan and meiotic chromosome segregation but had slightly reduced fertility. The mutant worms had reduced DNA damage-induced germ-line apoptosis after genotoxic stress. The msh-2 mutants also had elevated levels of microsatellite instability and increased rates of reversion of the dominant unc-58(e665) mutation. In addition, serially passaged cultures of msh-2 worms died out much more quickly than those of wild-type worms. These results demonstrate that msh-2 function in C. elegans is important in regulating both short- and long-term genomic stability.
59 downloads since deposited on 11 Feb 2008
15 downloads since 12 months
|Item Type:||Journal Article, refereed|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|Dewey Decimal Classification:||570 Life sciences; biology|
|Date:||19 February 2002|
|Deposited On:||11 Feb 2008 12:19|
|Last Modified:||28 Nov 2013 01:19|
|Publisher:||National Academy of Sciences|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page