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A new method to assess available chlorine in small volumes of liquid


Rechenberg, Dan-K; Ragnarsson, Kjartan T; Rüeger, Sina; Held, Leonhard; Mohn, Dirk; Zehnder, Matthias (2014). A new method to assess available chlorine in small volumes of liquid. Journal of Endodontics, 40(4):534-537.

Abstract

INTRODUCTION: There is no robust and simple way to quantify available chlorine from small volumes such as human root canals. Therefore, a new method was developed and assessed.
METHODS: Standardized size-40 paper points were soaked in a 15% (w/v) potassium iodide solution for 1 minute. Subsequently, the paper points were placed in an incubator and dried at 110°C for 4 hours. The paper points (n = 5 per measurement) were then dipped in different concentrations of NaOCl and photographed under standardized conditions in RAW format. The pictures were imported to image processing software and adjusted to the standardized background. The red, green, and blue levels of the paper points were assessed at a predefined area. Inverse regression was used to determine NaOCl concentration from red, green, and blue values, with both explanatory and outcome variables log-transformed to base 10.
RESULTS: The red value measurements were chosen for further analysis based on a comparison of the coefficient of determination (R(2)) and a residual analysis. The method was applied to concentrations of NaOCl between 0.0001% and 1% (R(2) = 0.92). In this range, NaOCI concentrations could be assessed with an error not larger than 3-fold the determined concentration.
CONCLUSIONS: The present method proved to be robust to determine the order of magnitude of available chlorine that is present in a small volume. This should be useful for endodontic research.

INTRODUCTION: There is no robust and simple way to quantify available chlorine from small volumes such as human root canals. Therefore, a new method was developed and assessed.
METHODS: Standardized size-40 paper points were soaked in a 15% (w/v) potassium iodide solution for 1 minute. Subsequently, the paper points were placed in an incubator and dried at 110°C for 4 hours. The paper points (n = 5 per measurement) were then dipped in different concentrations of NaOCl and photographed under standardized conditions in RAW format. The pictures were imported to image processing software and adjusted to the standardized background. The red, green, and blue levels of the paper points were assessed at a predefined area. Inverse regression was used to determine NaOCl concentration from red, green, and blue values, with both explanatory and outcome variables log-transformed to base 10.
RESULTS: The red value measurements were chosen for further analysis based on a comparison of the coefficient of determination (R(2)) and a residual analysis. The method was applied to concentrations of NaOCl between 0.0001% and 1% (R(2) = 0.92). In this range, NaOCI concentrations could be assessed with an error not larger than 3-fold the determined concentration.
CONCLUSIONS: The present method proved to be robust to determine the order of magnitude of available chlorine that is present in a small volume. This should be useful for endodontic research.

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3 citations in Web of Science®
2 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Epidemiology, Biostatistics and Prevention Institute (EBPI)
04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2014
Deposited On:22 May 2014 08:13
Last Modified:05 Apr 2016 17:52
Publisher:Elsevier
ISSN:0099-2399
Publisher DOI:https://doi.org/10.1016/j.joen.2013.10.015
PubMed ID:24666906
Permanent URL: https://doi.org/10.5167/uzh-95968

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