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Microscopy through turbid layers using wave front shaping


Ghielmetti, Giulia. Microscopy through turbid layers using wave front shaping. 2014, University of Zurich, Faculty of Science.

Abstract

Microscopy through turbid layers is of great interest for biological applications. We present a setup, based on wave front shaping, which allows to image a fluorescent structure behind a turbid layer at diffraction-limited resolution. In this work we address three main problems. We first propose two methods to create a focus behind a turbid layer: the first based on the direct feedback of a detector placed behind the turbid layer, while the second, without direct access, allows to focus on a fluorescent nanobead. We then show how to move the focus in three dimensions, through memory effect, allowing to obtain an image of the structure behind the turbid layer through the measurement of the fluorescence emitted. Finally we propose an experimental attempt to enhance the contours of the image, based on a second scanning performed with a ring shaped focus.

Microscopy through turbid layers is of great interest for biological applications. We present a setup, based on wave front shaping, which allows to image a fluorescent structure behind a turbid layer at diffraction-limited resolution. In this work we address three main problems. We first propose two methods to create a focus behind a turbid layer: the first based on the direct feedback of a detector placed behind the turbid layer, while the second, without direct access, allows to focus on a fluorescent nanobead. We then show how to move the focus in three dimensions, through memory effect, allowing to obtain an image of the structure behind the turbid layer through the measurement of the fluorescence emitted. Finally we propose an experimental attempt to enhance the contours of the image, based on a second scanning performed with a ring shaped focus.

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Additional indexing

Item Type:Dissertation
Referees:Aegerter C M, Keller H, Scheffold F
Communities & Collections:07 Faculty of Science > Physics Institute
Dewey Decimal Classification:530 Physics
Language:English
Date:5 June 2014
Deposited On:23 Jun 2014 14:28
Last Modified:05 Apr 2016 17:55
Number of Pages:70
Permanent URL: https://doi.org/10.5167/uzh-96676

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