Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-967

Gerlitz, O; Nellen, D; Ottiger, M; Basler, K (2002). A screen for genes expressed in Drosophila imaginal discs. International Journal of Developmental Biology, 46(1):173-176.

[img]
Preview
PDF
206kB

Abstract

The development of Drosophila imaginal discs serves as a model system to understand how genes determine the shape and size of an organ. The identification of genes involved in this process is an important step towards this goal. Here we describe a P-element based enhancer trap screen for genes expressed in the larval imaginal discs. Our aim was to establish a large collection of enhancer trap lines each showing expression of Gal4 in imaginal discs. To this end, we improved the well established P-element vector pGawB in order to obtain higher in vivo transposition frequencies. In addition we chose an F1-screening approach using UAS-GFP as a reporter gene. This system permits the efficient screening of larval and pupal stages of living animals and the detection of imaginal gene expression patterns through the transparent cuticle. The procedure has been optimized for high-throughput. 2'000 P-element insertions have been established which exhibit expression in imaginal discs.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:2002
Deposited On:11 Feb 2008 12:19
Last Modified:27 Nov 2013 20:51
Publisher:Universidad del Pais Vasco
ISSN:0214-6282
PubMed ID:11902680
Citations:Web of Science®. Times Cited: 24
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page