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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-9984

Zenhaeusern, G; Gubser, P; Eisele, P; Gasser, O; Steinhuber, A; Trampuz, A; Handschin, C; Luster, A D; Hess, C (2009). A high-mobility, low-cost phenotype defines human effector-memory CD8+ T cells. Blood, 113(1):95-99.

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T cells move randomly ("random-walk"), a characteristic thought to be integral to their function. Using migration assays and time-lapse microscopy, we found that CD8+ T cells lacking the lymph node homing receptors CCR7 and CD62L migrate more efficiently in transwell assays, and that these same cells are characterized by a high frequency of cells exhibiting random crawling activity under culture conditions mimicking the interstitial/extravascular milieu, but not when examined on endothelial cells. To assess the energy efficiency of cells crawling at a high frequency, we measured mRNA expression of genes key to mitochondrial energy metabolism (peroxisome proliferator-activated receptor gamma coactivator 1beta [PGC-1beta], estrogen-related receptor alpha [ERRalpha], cytochrome C, ATP synthase, and the uncoupling proteins [UCPs] UCP-2 and -3), quantified ATP contents, and performed calorimetric analyses. Together these assays indicated a high energy efficiency of the high crawling frequency CD8+ T-cell population, and identified differentially regulated heat production among nonlymphoid versus lymphoid homing CD8+ T cells.


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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Date:January 2009
Deposited On:15 Mar 2009 09:53
Last Modified:05 Apr 2016 12:49
Publisher:American Society of Hematology
Additional Information:This research was originally published in Blood, 2009 Jan 1;113(1):95-9. Epub 2008 Oct 9. Copyright by the American Society of Hematology
Publisher DOI:10.1182/blood-2008-04-153262
PubMed ID:18845792

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