Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-9984
Zenhaeusern, G; Gubser, P; Eisele, P; Gasser, O; Steinhuber, A; Trampuz, A; Handschin, C; Luster, A D; Hess, C (2009). A high-mobility, low-cost phenotype defines human effector-memory CD8+ T cells. Blood, 113(1):95-99.
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T cells move randomly ("random-walk"), a characteristic thought to be integral to their function. Using migration assays and time-lapse microscopy, we found that CD8+ T cells lacking the lymph node homing receptors CCR7 and CD62L migrate more efficiently in transwell assays, and that these same cells are characterized by a high frequency of cells exhibiting random crawling activity under culture conditions mimicking the interstitial/extravascular milieu, but not when examined on endothelial cells. To assess the energy efficiency of cells crawling at a high frequency, we measured mRNA expression of genes key to mitochondrial energy metabolism (peroxisome proliferator-activated receptor gamma coactivator 1beta [PGC-1beta], estrogen-related receptor alpha [ERRalpha], cytochrome C, ATP synthase, and the uncoupling proteins [UCPs] UCP-2 and -3), quantified ATP contents, and performed calorimetric analyses. Together these assays indicated a high energy efficiency of the high crawling frequency CD8+ T-cell population, and identified differentially regulated heat production among nonlymphoid versus lymphoid homing CD8+ T cells.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Center for Integrative Human Physiology|
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||15 Mar 2009 10:53|
|Last Modified:||23 Nov 2012 16:23|
|Publisher:||American Society of Hematology|
|Additional Information:||This research was originally published in Blood, 2009 Jan 1;113(1):95-9. Epub 2008 Oct 9. Copyright by the American Society of Hematology|
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