Header

UZH-Logo

Maintenance Infos

A new in vivo MRI method to non-invasively monitor and quantify the perfusion capacity of 3D-biomaterials grown on the chorioallantoic membrane of chick embryos


Kivrak Pfiffner, Fatma; Waschkies, Conny; Tian, Yinghua; Woloszyk, Anna; Calcagni, Maurizio; Giovanoli, Pietro; Rudin, Markus; Buschmann, Johanna (2015). A new in vivo MRI method to non-invasively monitor and quantify the perfusion capacity of 3D-biomaterials grown on the chorioallantoic membrane of chick embryos. Tissue engineering. Part C, Methods, 21(4):339-346.

Abstract

Adequate vascularization in biomaterials is essential for tissue regeneration and repair. Current models do not allow easy analysis of vascularization of implants in vivo, leaving it a highly desirable goal. A tool that allows to monitor the perfusion capacity of such biomaterials non-invasively in a cheap, efficient and reliable in vivo model would hence add great benefit to the research in this field. We established, for the first time, an in vivo Magnetic Resonance Imaging (MRI) method to quantify the perfusion capacity of a model biomaterial, DegraPol® foam scaffold, placed on the embryonic avian chorioallantoic membrane (CAM) in ovo. Perfusion capacity was assessed through changes in the longitudinal relaxation rate before and after injection of a paramagnetic MRI contrast agent, Gd-DOTA (®Dotarem, Guerbet S.A.). Relaxation rate changes were compared in three different regions of the scaffold, i.e. at the interface to the CAM, in the middle and on the surface of the scaffold (p < 0.05). The highest relaxation rate changes, and hence perfusion capacities, were measured in the interface region where the scaffold was attached to the CAM, whereas the surface of the scaffold showed the lowest relaxation rate changes. A strong positive correlation was obtained between relaxation rate changes and histologically determined vessel density (R2 = 0.983), which corroborates our MRI findings. As a proof-of-principle, we measured the perfusion capacity in different scaffold materials, silk fibroin either with or without human dental pulp stem cells. For these, 3 - 4 times larger perfusion capacities were obtained compared to DegraPol®; demonstrating that our method is sensitive to reveal such differences. In summary, we present a novel in vivo method for analyzing the perfusion capacity in 3D- biomaterials grown on the CAM, enabling the determination of the perfusion capacity of a large variety of bioengineered materials.

Abstract

Adequate vascularization in biomaterials is essential for tissue regeneration and repair. Current models do not allow easy analysis of vascularization of implants in vivo, leaving it a highly desirable goal. A tool that allows to monitor the perfusion capacity of such biomaterials non-invasively in a cheap, efficient and reliable in vivo model would hence add great benefit to the research in this field. We established, for the first time, an in vivo Magnetic Resonance Imaging (MRI) method to quantify the perfusion capacity of a model biomaterial, DegraPol® foam scaffold, placed on the embryonic avian chorioallantoic membrane (CAM) in ovo. Perfusion capacity was assessed through changes in the longitudinal relaxation rate before and after injection of a paramagnetic MRI contrast agent, Gd-DOTA (®Dotarem, Guerbet S.A.). Relaxation rate changes were compared in three different regions of the scaffold, i.e. at the interface to the CAM, in the middle and on the surface of the scaffold (p < 0.05). The highest relaxation rate changes, and hence perfusion capacities, were measured in the interface region where the scaffold was attached to the CAM, whereas the surface of the scaffold showed the lowest relaxation rate changes. A strong positive correlation was obtained between relaxation rate changes and histologically determined vessel density (R2 = 0.983), which corroborates our MRI findings. As a proof-of-principle, we measured the perfusion capacity in different scaffold materials, silk fibroin either with or without human dental pulp stem cells. For these, 3 - 4 times larger perfusion capacities were obtained compared to DegraPol®; demonstrating that our method is sensitive to reveal such differences. In summary, we present a novel in vivo method for analyzing the perfusion capacity in 3D- biomaterials grown on the CAM, enabling the determination of the perfusion capacity of a large variety of bioengineered materials.

Statistics

Altmetrics

Downloads

69 downloads since deposited on 04 Nov 2014
21 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology

04 Faculty of Medicine > Institute of Biomedical Engineering
04 Faculty of Medicine > University Hospital Zurich > Clinic for Reconstructive Surgery
Dewey Decimal Classification:570 Life sciences; biology
170 Ethics
610 Medicine & health
Language:English
Date:2015
Deposited On:04 Nov 2014 08:43
Last Modified:08 Dec 2017 07:49
Publisher:Mary Ann Liebert
ISSN:1937-3384
Additional Information:This is a copy of an article published in Tissue engineering. Part C, Methods © 2014 [copyright Mary Ann Liebert, Inc.]; Tissue engineering. Part C, Methods is available online at: http://www.liebertonline.com.
Publisher DOI:https://doi.org/10.1089/ten.TEC.2014.0212
PubMed ID:25266825

Download

Download PDF  'A new in vivo MRI method to non-invasively monitor and quantify the perfusion capacity of 3D-biomaterials grown on the chorioallantoic membrane of chick embryos'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 3MB
View at publisher