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Applicability of common methods for short time erosion analysis in vitro


Hannig, C; Becker, K; Yankeu-Ngalene, V E; Attin, T (2008). Applicability of common methods for short time erosion analysis in vitro. Oral Health & Preventive Dentistry, 6(3):239-248.

Abstract

PURPOSE: Dental erosion can be measured by different methods. The aim of the present study was to check the applicability of common methods to determine initial erosive effects. MATERIALS AND METHODS: Enamel surfaces (4.5 mm2) were eroded in vitro by treatment with hydrochloric acid (pH 2, 2.3 and 2.6) for 5 to 60 s or 240 s, respectively. Erosive effects were assayed with three different methods: Knoop's diamond indentation, profilometry and the determination of the dissolved calcium ions (Ca2+) in a colorimetric assay based on the arsenazo-III-reaction. RESULTS: Erosive mineral loss of > 1 microm are measurable with profilometry. This corresponds to the erosive effects that occur after 60 s or more. Profilometric data yielded variance of up to 50%. Knoop's diamond indentation also showed some limitations: the depth of indentation reached a plateau after 30 to 120 s and the measurements showed variance of up to 85%. With the colorimetric assay, short time erosive effects occurring within 5 s could be assessed precisely and kinetically. The method allowed small amounts of 400 pmol Ca2+ per well to be quantified in small volumes with little variability. CONCLUSIONS: For evaluation and quantification of short time erosive effects, the colorimetric method is superior to diamond indentation and profilometry.

Abstract

PURPOSE: Dental erosion can be measured by different methods. The aim of the present study was to check the applicability of common methods to determine initial erosive effects. MATERIALS AND METHODS: Enamel surfaces (4.5 mm2) were eroded in vitro by treatment with hydrochloric acid (pH 2, 2.3 and 2.6) for 5 to 60 s or 240 s, respectively. Erosive effects were assayed with three different methods: Knoop's diamond indentation, profilometry and the determination of the dissolved calcium ions (Ca2+) in a colorimetric assay based on the arsenazo-III-reaction. RESULTS: Erosive mineral loss of > 1 microm are measurable with profilometry. This corresponds to the erosive effects that occur after 60 s or more. Profilometric data yielded variance of up to 50%. Knoop's diamond indentation also showed some limitations: the depth of indentation reached a plateau after 30 to 120 s and the measurements showed variance of up to 85%. With the colorimetric assay, short time erosive effects occurring within 5 s could be assessed precisely and kinetically. The method allowed small amounts of 400 pmol Ca2+ per well to be quantified in small volumes with little variability. CONCLUSIONS: For evaluation and quantification of short time erosive effects, the colorimetric method is superior to diamond indentation and profilometry.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2008
Deposited On:16 Jan 2009 08:12
Last Modified:06 Dec 2017 16:55
Publisher:Quintessence Publishing
ISSN:1602-1622
Official URL:http://www.quintpub.com/journals/ohpd/archive_display_abstract.php3?journalArt=8298
Related URLs:http://www.quintpub.com/ (Publisher)
PubMed ID:19119579

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