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Hydrogen peroxide enhances enterokinase-catalysed proteolytic cleavage of fusion protein


Cui, T; Gao, Y; Ang, C X; Puah, C M; Gutte, B; Lam, Y (2008). Hydrogen peroxide enhances enterokinase-catalysed proteolytic cleavage of fusion protein. Recent Patents on Biotechnology, 2(3):189-190.

Abstract

The effects of hydrogen peroxide on enterokinase catalysis were studied using several fusion proteins recombinantly produced from E. coli. It was demonstrated that hydrogen peroxide enhanced the rate of enterokinase cleavage reaction, leading to a faster release of the target peptide as discussed in patent WO07149053. Among the conditions tested, we observed that hydrogen peroxide could exert its effect on the cleavage of fusion proteins over a wide range of pH and temperature. This finding might provide a simple solution for the accelerated enterokinase cleavage of thermolabile fusion proteins at low temperature.

Abstract

The effects of hydrogen peroxide on enterokinase catalysis were studied using several fusion proteins recombinantly produced from E. coli. It was demonstrated that hydrogen peroxide enhanced the rate of enterokinase cleavage reaction, leading to a faster release of the target peptide as discussed in patent WO07149053. Among the conditions tested, we observed that hydrogen peroxide could exert its effect on the cleavage of fusion proteins over a wide range of pH and temperature. This finding might provide a simple solution for the accelerated enterokinase cleavage of thermolabile fusion proteins at low temperature.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:23 October 2008
Deposited On:23 Jan 2009 15:52
Last Modified:05 Apr 2016 12:50
Publisher:Bentham Science
ISSN:1872-2083
Publisher DOI:https://doi.org/10.2174/187220808786240971
PubMed ID:19075866

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