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A Link between Arabinose Utilization and Oxalotrophy in Bradyrhizobium japonicum


Koch, M; Delmotte, N; Ahrens, C H; Omasits, U; Schneider, K; Danza, F; Padhi, B; Murset, V; Braissant, O; Vorholt, J A; Hennecke, H; Pessi, G (2014). A Link between Arabinose Utilization and Oxalotrophy in Bradyrhizobium japonicum. Applied and Environmental Microbiology, 80(7):2094-2101.

Abstract

Rhizobia have a versatile catabolism that allows them to compete successfully with other microorganisms for nutrients in the soil and in the rhizosphere of their respective host plants. In this study, Bradyrhizobium japonicum USDA 110 was found to be able to utilize oxalate as the sole carbon source. A proteome analysis of cells grown in minimal medium containing arabinose suggested that oxalate oxidation extends the arabinose degradation branch via glycolaldehyde. A mutant of the key pathway genes oxc (for oxalyl-coenzyme A decarboxylase) and frc (for formyl-coenzyme A transferase) was constructed and shown to be (i) impaired in growth on arabinose and (ii) unable to grow on oxalate. Oxalate was detected in roots and, at elevated levels, in root nodules of four different B. japonicum host plants. Mixed-inoculation experiments with wild-type and oxc-frc mutant cells revealed that oxalotrophy might be a beneficial trait of B. japonicum at some stage during legume root nodule colonization.

Abstract

Rhizobia have a versatile catabolism that allows them to compete successfully with other microorganisms for nutrients in the soil and in the rhizosphere of their respective host plants. In this study, Bradyrhizobium japonicum USDA 110 was found to be able to utilize oxalate as the sole carbon source. A proteome analysis of cells grown in minimal medium containing arabinose suggested that oxalate oxidation extends the arabinose degradation branch via glycolaldehyde. A mutant of the key pathway genes oxc (for oxalyl-coenzyme A decarboxylase) and frc (for formyl-coenzyme A transferase) was constructed and shown to be (i) impaired in growth on arabinose and (ii) unable to grow on oxalate. Oxalate was detected in roots and, at elevated levels, in root nodules of four different B. japonicum host plants. Mixed-inoculation experiments with wild-type and oxc-frc mutant cells revealed that oxalotrophy might be a beneficial trait of B. japonicum at some stage during legume root nodule colonization.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:April 2014
Deposited On:12 Feb 2015 13:04
Last Modified:16 Jul 2017 06:03
Publisher:American Society for Microbiology
ISSN:0099-2240
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1128/AEM.03314-13
Related URLs:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993136/
PubMed ID:24463964

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