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Direct imaging of fluorescent structures behind turbid layers


Ghielmetti, Giulia; Aegerter, Christof M (2014). Direct imaging of fluorescent structures behind turbid layers. Optics Express, 22(2):1981.

Abstract

We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the optimization leads to the focusing on a single particle whose surroundings are subsequently scanned. In combination with a parabolic phase pattern, this method can be extended to a three dimensional imaging method inside turbid media.

Abstract

We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the optimization leads to the focusing on a single particle whose surroundings are subsequently scanned. In combination with a parabolic phase pattern, this method can be extended to a three dimensional imaging method inside turbid media.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Physics Institute
Dewey Decimal Classification:530 Physics
Language:English
Date:2014
Deposited On:23 Jan 2015 14:02
Last Modified:27 Apr 2017 23:15
Publisher:Optical Society of America
Series Name:Optics Express
Number of Pages:8
ISSN:1094-4087
Additional Information:This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-22-2-1981. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
Publisher DOI:https://doi.org/10.1364/OE.22.001981
Official URL:http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-22-2-1981

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