Header

UZH-Logo

Maintenance Infos

In vitro evaluation of a novel biofilm remover


Hofer, D; Meier, A; Sener, B; Guggenheim, B; Attin, T; Schmidlin, P R (2015). In vitro evaluation of a novel biofilm remover. International Journal of Dental Hygiene, 13(4):246-253.

Abstract

OBJECTIVE: To evaluate a novel device for its efficacy in removing experimental biofilm from root surfaces and its potential for concomitantly removing/roughening the surface substance.
METHODS AND MATERIALS: A novel acrylic rotary device (biofilm remover, BR) was tested in vitro in three experiments: surface loss, surface roughness [positive controls: Perioset (PS) and Proxoshape (PR)] and biofilm removal [positive controls: ultrasonic (US) and PS]. Surface loss/surface roughness was evaluated for dentin samples instrumented for three 20 s periods. The calcium removed during instrumentation was analysed after each interval and cumulatively, using atomic absorption spectrophotometry (AAS). Surface roughness was measured using profilometric analysis. Biofilm removal was evaluated on dentin specimens coated with a 64.5 h 6-species in vitro formed biofilm, after one 20 s treatment. Surface loss was analysed using anova with Scheffé post hoc test, and surface roughness/biofilm removal was analysed using Mann-Whitney test (all P ≤ 0.05).
RESULTS: Significantly less substance loss [μg (±1 SD)] was observed with the novel device at all time points, both interval and cumulative (1.0 (±0.5) versus 9.3 (±3.2) PS and 9.9 (±1.9) PR at 60 s). Surface roughness [μm (95% CI)] was significantly lower for BR than for PS and PR [0.00 (-0.01, 0.08) 0.20 (0.16, 0.27) and 0.21 (0.19, 0.24) at 60 s]. Significantly less biofilm bacteria remained after treatment with both BR 4.5 (-0.1, 16.2) and US 1.9 (-0.2, 14.3), compared to PS 52 (27.9, 82.1).
CONCLUSIONS: The novel biofilm remover was less damaging to dentin surfaces, while removing biofilm at least as effectively as devices used in this study.

Abstract

OBJECTIVE: To evaluate a novel device for its efficacy in removing experimental biofilm from root surfaces and its potential for concomitantly removing/roughening the surface substance.
METHODS AND MATERIALS: A novel acrylic rotary device (biofilm remover, BR) was tested in vitro in three experiments: surface loss, surface roughness [positive controls: Perioset (PS) and Proxoshape (PR)] and biofilm removal [positive controls: ultrasonic (US) and PS]. Surface loss/surface roughness was evaluated for dentin samples instrumented for three 20 s periods. The calcium removed during instrumentation was analysed after each interval and cumulatively, using atomic absorption spectrophotometry (AAS). Surface roughness was measured using profilometric analysis. Biofilm removal was evaluated on dentin specimens coated with a 64.5 h 6-species in vitro formed biofilm, after one 20 s treatment. Surface loss was analysed using anova with Scheffé post hoc test, and surface roughness/biofilm removal was analysed using Mann-Whitney test (all P ≤ 0.05).
RESULTS: Significantly less substance loss [μg (±1 SD)] was observed with the novel device at all time points, both interval and cumulative (1.0 (±0.5) versus 9.3 (±3.2) PS and 9.9 (±1.9) PR at 60 s). Surface roughness [μm (95% CI)] was significantly lower for BR than for PS and PR [0.00 (-0.01, 0.08) 0.20 (0.16, 0.27) and 0.21 (0.19, 0.24) at 60 s]. Significantly less biofilm bacteria remained after treatment with both BR 4.5 (-0.1, 16.2) and US 1.9 (-0.2, 14.3), compared to PS 52 (27.9, 82.1).
CONCLUSIONS: The novel biofilm remover was less damaging to dentin surfaces, while removing biofilm at least as effectively as devices used in this study.

Statistics

Altmetrics

Downloads

17 downloads since deposited on 30 Jan 2015
8 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2015
Deposited On:30 Jan 2015 09:26
Last Modified:21 Nov 2017 17:48
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:1601-5029
Additional Information:This is the accepted version of the following article: [Int J Dent Hygiene DOI: 10.1111/idh.12113 Hofer D, Meier A, Sener B, Guggenheim B, Attin T, Schmidlin PR. In vitro evaluation of a novel biofilm remover.], which has been published in final form at [http://onlinelibrary.wiley.com/doi/10.1111/idh.12113/abstract].
Publisher DOI:https://doi.org/10.1111/idh.12113
PubMed ID:25421848

Download

Download PDF  'In vitro evaluation of a novel biofilm remover'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 7MB
View at publisher