Header

UZH-Logo

Maintenance Infos

Destruction of a distal hypoxia response element abolishes trans-activation of the PAG1 gene mediated by HIF-independent chromatin looping


Schörg, Alexandra Sabine; Santambrogio, Sara; Platt, James L; Schödel, Johannes; Lindenmeyer, Maja T; Cohen, Clemens D; Schrödter, Katrin; Mole, David R; Wenger, Roland H; Hoogewijs, David (2015). Destruction of a distal hypoxia response element abolishes trans-activation of the PAG1 gene mediated by HIF-independent chromatin looping. Nucleic Acids Research:1-15.

Abstract

A crucial step in the cellular adaptation to oxygen deficiency is the binding of hypoxia-inducible factors (HIFs) to hypoxia response elements (HREs) of oxygen-regulated genes. Genome-wide HIF- 1alpha/2alpha/Beta DNA-binding studies revealed that the majority of HREs reside distant to the promoter regions, but the function of these distal HREs has only been marginally studied in the genomic context. We used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to localize and functionally characterize a 82 kb upstream HRE that solely drives oxygen-regulated expression of the newly identified HIF target gene PAG1. PAG1, a transmembrane adaptor protein involved in Src signalling, was hypoxically induced in various cell lines andmouse tissues. ChIP and reporter gene assays demonstrated that the −82 kb HRE regulates PAG1, but not an equally distant gene further upstream, by direct interaction with HIF. Ablation of the consensus HRE motif abolished the hypoxic induction of PAG1 but not general oxygen signalling. 3C assays revealed that the −82 kb HRE physically associates with thePAG1 promoter region, independent of HIF-DNA interaction. These results demonstrate a constitutive interaction between the −82 kb HRE and the PAG1 promoter, suggesting a physiologically important rapid response to hypoxia.

Abstract

A crucial step in the cellular adaptation to oxygen deficiency is the binding of hypoxia-inducible factors (HIFs) to hypoxia response elements (HREs) of oxygen-regulated genes. Genome-wide HIF- 1alpha/2alpha/Beta DNA-binding studies revealed that the majority of HREs reside distant to the promoter regions, but the function of these distal HREs has only been marginally studied in the genomic context. We used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to localize and functionally characterize a 82 kb upstream HRE that solely drives oxygen-regulated expression of the newly identified HIF target gene PAG1. PAG1, a transmembrane adaptor protein involved in Src signalling, was hypoxically induced in various cell lines andmouse tissues. ChIP and reporter gene assays demonstrated that the −82 kb HRE regulates PAG1, but not an equally distant gene further upstream, by direct interaction with HIF. Ablation of the consensus HRE motif abolished the hypoxic induction of PAG1 but not general oxygen signalling. 3C assays revealed that the −82 kb HRE physically associates with thePAG1 promoter region, independent of HIF-DNA interaction. These results demonstrate a constitutive interaction between the −82 kb HRE and the PAG1 promoter, suggesting a physiologically important rapid response to hypoxia.

Statistics

Citations

Dimensions.ai Metrics
4 citations in Web of Science®
4 citations in Scopus®
3 citations in Microsoft Academic
Google Scholar™

Altmetrics

Downloads

30 downloads since deposited on 27 May 2015
4 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2015
Deposited On:27 May 2015 12:34
Last Modified:18 Apr 2018 11:46
Publisher:Oxford University Press
ISSN:0305-1048
Funders:Forschungskredit, University of Zürich, Sassella Stiftung, Zürich, Flemish FWO, postdoctoral fellowship, European Commission, Marie Curie IEF Postdoctoral Fellowship, State Secretariat of Education, Research and Innovation, Bern, Switzerland, KFSP Tumor Oxygenation of the University of Z¨urich, Swiss National Science Foundation (31003A 146203), Wellcome Trust (088182/Z/09/Z), Biobank ERCBKFB (Else Kröner-Fresenius-Foundation), Funding for open access charge: University of Zurich
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/nar/gkv506
Project Information:
  • : Funder
  • : Grant ID
  • : Project TitleForschungskredit, University of Zürich
  • : Funder
  • : Grant ID
  • : Project TitleSassella Stiftung, Zürich
  • : Funder
  • : Grant ID
  • : Project TitleFlemish FWO, postdoctoral fellowship
  • : Funder
  • : Grant ID
  • : Project TitleEuropean Commission, Marie Curie IEF Postdoctoral Fellowship
  • : Funder
  • : Grant ID
  • : Project TitleState Secretariat of Education, Research and Innovation, Bern, Switzerland
  • : Funder
  • : Grant ID
  • : Project TitleKFSP Tumor Oxygenation of the University of Z¨urich
  • : FunderSNSF
  • : Grant ID
  • : Project TitleSwiss National Science Foundation (31003A 146203)
  • : Funder
  • : Grant ID
  • : Project TitleWellcome Trust (088182/Z/09/Z)
  • : Funder
  • : Grant ID
  • : Project TitleBiobank ERCBKFB (Else Kröner-Fresenius-Foundation)
  • : Funder
  • : Grant ID
  • : Project TitleFunding for open access charge: University of Zurich

Download

Download PDF  'Destruction of a distal hypoxia response element abolishes trans-activation of the PAG1 gene mediated by HIF-independent chromatin looping'.
Preview
Content: Published Version
Filetype: PDF
Size: 4MB
View at publisher