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Fluorescence Tracking of Genome Release during the Mechanical Unpacking of Single Viruses


Ortega-Esteban, Alvaro; Bodensiek, Kai; San Martín, Carmen; Suomalainen, Maarit; Greber, Urs F; de Pablo, Pedro J; Schaap, Iwan A T (2015). Fluorescence Tracking of Genome Release during the Mechanical Unpacking of Single Viruses. ACS Nano:1-28.

Abstract

Viruses package their genome in a robust protein coat to protect it during transmission between cells and organisms. In a reaction termed uncoating, the virus is progressively weakened during entry into cells. At the end of the uncoating process the genome separates, becomes transcriptionally active, and initiates the production of progeny. Here, we triggered the disruption of single human adenovirus capsids with atomic force microscopy, and followed genome exposure by single molecule fluorescence microscopy. This method allowed the comparison of immature (non-infectious) and mature (infectious) adenovirus particles. We observed two condensation states of the fluorescently labeled genome, a feature of the virus that may be related to infectivity. Beyond tracking the unpacking of virus genomes this approach may find application in testing the cargo release of bio-inspired delivery vehicles.

Abstract

Viruses package their genome in a robust protein coat to protect it during transmission between cells and organisms. In a reaction termed uncoating, the virus is progressively weakened during entry into cells. At the end of the uncoating process the genome separates, becomes transcriptionally active, and initiates the production of progeny. Here, we triggered the disruption of single human adenovirus capsids with atomic force microscopy, and followed genome exposure by single molecule fluorescence microscopy. This method allowed the comparison of immature (non-infectious) and mature (infectious) adenovirus particles. We observed two condensation states of the fluorescently labeled genome, a feature of the virus that may be related to infectivity. Beyond tracking the unpacking of virus genomes this approach may find application in testing the cargo release of bio-inspired delivery vehicles.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:18 September 2015
Deposited On:24 Sep 2015 13:13
Last Modified:24 Nov 2017 03:10
Publisher:American Chemical Society (ACS)
ISSN:1936-0851
Publisher DOI:https://doi.org/10.1021/acsnano.5b03020
PubMed ID:26381363

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