Header

UZH-Logo

Maintenance Infos

Metabolism gene signatures and surgical site infections in abdominal surgery


Kong, Bo; Bruns, Philipp; Raulefs, Susanne; Rieder, Simon; Paul, Laura; Prazeresda Costa, Olivia; Buch, Thorsten; Theis, Fabian J; Michalski, Christoph W; Kleeff, Jörg (2015). Metabolism gene signatures and surgical site infections in abdominal surgery. International Journal of Surgery, 14:67-74.

Abstract

Introduction: Surgical site infections (SSI) represent a significant cause of morbidity in abdominal surgery. The objective of this study was to determine the gene expression signature in subcutaneous tissues in relation to SSI.
Methods: To determine differences in gene expression, microarray analysis were performed from bulk tissue mRNA of subcutaneous tissues prospectively collected in 92 patients during open abdominal surgery. 10 patients (11%) developed incisional (superficial and deep) SSI.
Results: Preoperative risk factors in patients with SSI were not significantly different from those in patients
without wound infections. 1025 genes were differentially expressed between the groups, of which the AZGP1 and ALDH1A3 genes were the highest down- and upregulated ones. Hierarchical clustering demonstrated strong similarity within the respective groups (SSI vs. no-SSI) indicating inter-group distinctness. In a functional classification, genes controlling cell metabolism were mostly downregulated in subcutaneous tissues of patients that subsequently developed SSI.
Conclusion: Altered expression of metabolism genes in subcutaneous tissues might constitute a risk factor for postoperative abdominal SSI.

Abstract

Introduction: Surgical site infections (SSI) represent a significant cause of morbidity in abdominal surgery. The objective of this study was to determine the gene expression signature in subcutaneous tissues in relation to SSI.
Methods: To determine differences in gene expression, microarray analysis were performed from bulk tissue mRNA of subcutaneous tissues prospectively collected in 92 patients during open abdominal surgery. 10 patients (11%) developed incisional (superficial and deep) SSI.
Results: Preoperative risk factors in patients with SSI were not significantly different from those in patients
without wound infections. 1025 genes were differentially expressed between the groups, of which the AZGP1 and ALDH1A3 genes were the highest down- and upregulated ones. Hierarchical clustering demonstrated strong similarity within the respective groups (SSI vs. no-SSI) indicating inter-group distinctness. In a functional classification, genes controlling cell metabolism were mostly downregulated in subcutaneous tissues of patients that subsequently developed SSI.
Conclusion: Altered expression of metabolism genes in subcutaneous tissues might constitute a risk factor for postoperative abdominal SSI.

Statistics

Citations

2 citations in Web of Science®
2 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 10 Nov 2015
1 download since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Laboratory Animal Science
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:2015
Deposited On:10 Nov 2015 15:17
Last Modified:05 Apr 2016 19:30
Publisher:Elsevier
ISSN:1743-9159
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.ijsu.2015.01.009
PubMed ID:25599917

Download