Header

UZH-Logo

Maintenance Infos

Trajectories of cell-cycle progression from fixed cell populations


Gut, Gabriele; Tadmor, Michelle D; Pe'er, Dana; Pelkmans, Lucas; Liberali, Prisca (2015). Trajectories of cell-cycle progression from fixed cell populations. Nature Methods, 12(10):951-954.

Abstract

An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.

Abstract

An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.

Statistics

Citations

16 citations in Web of Science®
14 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

44 downloads since deposited on 10 Dec 2015
29 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:24 August 2015
Deposited On:10 Dec 2015 08:52
Last Modified:08 Dec 2017 15:26
Publisher:Nature Publishing Group
ISSN:1548-7091
Publisher DOI:https://doi.org/10.1038/nmeth.3545
PubMed ID:26301842

Download

Download PDF  'Trajectories of cell-cycle progression from fixed cell populations'.
Preview
Content: Published Version
Language: English
Filetype: PDF
Size: 1MB
View at publisher