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Fast infrared spectroscopy of protein dynamics: advancing sensitivity and selectivity


Koziol, Klemens L; Johnson, Philip J M; Stucki-Buchli, Brigitte; Waldauer, Steven A; Hamm, Peter (2015). Fast infrared spectroscopy of protein dynamics: advancing sensitivity and selectivity. Current Opinion in Structural Biology, 34:1-6.

Abstract

2D-IR spectroscopy has matured to a powerful technique to study the structure and dynamics of peptides, but its extension to larger proteins is still in its infancy, the major limitations being sensitivity and selectivity. Site-selective information requires measuring single
vibrational probes at sub-millimolar concentrations where most proteins are still stable, which is a severe challenge for conventional (FT)IR spectroscopy. Besides its ultrafast time-resolution, a so far largely underappreciated potential of 2D-IR spectroscopy lies in its sensitivity gain. The present paper sets the goals and outlines strategies how to use that sensitivity gain together with properly designed vibrational labels to make IR spectroscopy a versatile tool to study a wide class of proteins.

Abstract

2D-IR spectroscopy has matured to a powerful technique to study the structure and dynamics of peptides, but its extension to larger proteins is still in its infancy, the major limitations being sensitivity and selectivity. Site-selective information requires measuring single
vibrational probes at sub-millimolar concentrations where most proteins are still stable, which is a severe challenge for conventional (FT)IR spectroscopy. Besides its ultrafast time-resolution, a so far largely underappreciated potential of 2D-IR spectroscopy lies in its sensitivity gain. The present paper sets the goals and outlines strategies how to use that sensitivity gain together with properly designed vibrational labels to make IR spectroscopy a versatile tool to study a wide class of proteins.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:17 April 2015
Deposited On:15 Dec 2015 11:29
Last Modified:01 Nov 2016 01:01
Publisher:Elsevier
ISSN:0959-440X
Funders:ERC advanced investigator grant (DYNALLO), Swiss National Science foundation through the NCCR MUST
Publisher DOI:https://doi.org/10.1016/j.sbi.2015.03.012
PubMed ID:25900180

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