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Faradic Peaks Enhanced by Carbon Nanotubes in Microsomal Cytochrome P450 Electrodes


Baj-Rossi, C; Müller, C; von Mandach, U; De Micheli, G; Carrara, S (2015). Faradic Peaks Enhanced by Carbon Nanotubes in Microsomal Cytochrome P450 Electrodes. Electroanalysis, 27(6):1507-1515.

Abstract

In this work we present an investigation on the behavior of microsomes containing human cytochrome P450 in cyclic voltammetry for drug detection. The microsomes are adsorbed on the surface of multi-walled carbon nanotubes by drop-casting. We demonstrate that the hydrophobic and highly electroactive surface of multi-walled carbon nanotubes enables to distinguish more clearly the contributions in reduction peak current attributed to the enzymatic components of microsomes. Voltammetric measurements were performed under several experimental conditions with two cytochrome P450-isoforms, 1A2 and 3A4. We show that the reduction current for the component of cytochrome P450-microsome linearly increases in the presence of a substrate.

Abstract

In this work we present an investigation on the behavior of microsomes containing human cytochrome P450 in cyclic voltammetry for drug detection. The microsomes are adsorbed on the surface of multi-walled carbon nanotubes by drop-casting. We demonstrate that the hydrophobic and highly electroactive surface of multi-walled carbon nanotubes enables to distinguish more clearly the contributions in reduction peak current attributed to the enzymatic components of microsomes. Voltammetric measurements were performed under several experimental conditions with two cytochrome P450-isoforms, 1A2 and 3A4. We show that the reduction current for the component of cytochrome P450-microsome linearly increases in the presence of a substrate.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2015
Deposited On:18 Dec 2015 10:06
Last Modified:29 Apr 2016 07:05
Publisher:Wiley-VCH Verlag
ISSN:1040-0397
Publisher DOI:https://doi.org/10.1002/elan.201400726

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