Header

UZH-Logo

Maintenance Infos

In vivo evidence for a lactate gradient from astrocytes to neurons


Abstract

The determination of lactate dynamics in brain tissue represents a challenge, partly because in vivo data at cellular resolution are not available. Here we monitored lactate in astrocytes and neurons of the primary somatosensory cortex of mice using the genetically-encoded FRET sensor Laconic in combination with two-photon laser scanning microscopy. An intravenous lactate injection rapidly increased the Laconic signal in both astrocytes and neurons, demonstrating high lactate permeability across the tissue. The signal increase was significantly smaller in astrocytes pointing to higher basal lactate levels in these cells, confirmed by a one-point in vivo calibration protocol.Trans-acceleration of the monocarboxylate transporter with pyruvate was able to reduce intracellular lactate in astrocytes but not in neurons. Collectively, this data provides in vivo evidence for a lactate gradient from astrocytes to neurons.

Abstract

The determination of lactate dynamics in brain tissue represents a challenge, partly because in vivo data at cellular resolution are not available. Here we monitored lactate in astrocytes and neurons of the primary somatosensory cortex of mice using the genetically-encoded FRET sensor Laconic in combination with two-photon laser scanning microscopy. An intravenous lactate injection rapidly increased the Laconic signal in both astrocytes and neurons, demonstrating high lactate permeability across the tissue. The signal increase was significantly smaller in astrocytes pointing to higher basal lactate levels in these cells, confirmed by a one-point in vivo calibration protocol.Trans-acceleration of the monocarboxylate transporter with pyruvate was able to reduce intracellular lactate in astrocytes but not in neurons. Collectively, this data provides in vivo evidence for a lactate gradient from astrocytes to neurons.

Statistics

Citations

31 citations in Web of Science®
31 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

54 downloads since deposited on 14 Jan 2016
34 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology

04 Faculty of Medicine > Neuroscience Center Zurich
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2016
Deposited On:14 Jan 2016 09:48
Last Modified:28 Apr 2017 02:38
Publisher:Cell Press (Elsevier)
ISSN:1550-4131
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.cmet.2015.10.010
PubMed ID:26698914

Download

Download PDF  'In vivo evidence for a lactate gradient from astrocytes to neurons'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 8MB
View at publisher