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Evaluation of the CHROMagarTM STEC medium for the detection of Shiga toxin-producing Escherichia coli


Cernela, Nicole; Zweifel, Claudio; Stephan, Roger (2015). Evaluation of the CHROMagarTM STEC medium for the detection of Shiga toxin-producing Escherichia coli. Journal of Food Safety and Food Quality, 66(2):28-32.

Abstract

Fast and reliable isolation of Shiga toxin-producing Escherichia coli (STEC) remains a challenge in routine diagnostics. The present study evaluated the performance of the CHROMagar™ STEC medium by testing the growth capacity of 39 STEC strains from human patients and of 35 non-target strains. The majority (83.3 %) of the 18 STEC strains belonging to the top-five serogroups (O26, O103, O111, O145, O157) did grow on and showed typical mauve colonies (exceptions were one O26 isolate and two sorbitol-fermenting non-motile O157 isolates). However, only five of the 21 STEC strains not belonging to the top-five serogroups showed growth and typical colonies. Of the 28 stx-negative E. coli strains (including 13 O157 isolates) and the seven nonE. coli strains, 10 stx-negative/eae-positive E. coli strains of serogroups O2, O26, O113, O128, O145 and O177 did grow and showed typical colonies. Thus, the CHROMagar™ STEC medium can not be recommended as a primary STEC screening method in routine diagnostics. The CHROMagar™ STEC medium should therefore only be used for specific questions or in routine STEC diagnostics in combination with another method or medium

Abstract

Fast and reliable isolation of Shiga toxin-producing Escherichia coli (STEC) remains a challenge in routine diagnostics. The present study evaluated the performance of the CHROMagar™ STEC medium by testing the growth capacity of 39 STEC strains from human patients and of 35 non-target strains. The majority (83.3 %) of the 18 STEC strains belonging to the top-five serogroups (O26, O103, O111, O145, O157) did grow on and showed typical mauve colonies (exceptions were one O26 isolate and two sorbitol-fermenting non-motile O157 isolates). However, only five of the 21 STEC strains not belonging to the top-five serogroups showed growth and typical colonies. Of the 28 stx-negative E. coli strains (including 13 O157 isolates) and the seven nonE. coli strains, 10 stx-negative/eae-positive E. coli strains of serogroups O2, O26, O113, O128, O145 and O177 did grow and showed typical colonies. Thus, the CHROMagar™ STEC medium can not be recommended as a primary STEC screening method in routine diagnostics. The CHROMagar™ STEC medium should therefore only be used for specific questions or in routine STEC diagnostics in combination with another method or medium

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Additional indexing

Other titles:Evaluierung des CHROMagar™ STEC Mediums für den Nachweis von Shigatoxin-bildenden Escherichia coli
Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:CHROMagar™ STEC, Shiga toxin-producing E. coli, serotypes, growth capacity, colony morphology
Language:English
Date:2015
Deposited On:18 Feb 2016 12:05
Last Modified:05 Apr 2016 20:05
Publisher:Schlütersche Verlagsgesellschaft
ISSN:0003-925X
Publisher DOI:https://doi.org/10.2376/0003-925X-66-28

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