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Sequence and partial functional analysis of canine Bcl-2 family proteins


de Brot, S; Schade, B; Croci, M; Dettwiler, M; Guscetti, F (2016). Sequence and partial functional analysis of canine Bcl-2 family proteins. Research in Veterinary Science, 104:126-135.

Abstract

Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy.We have assessed the degree of conservation of a set of canine Bcl-2 familymemberswith the human andmurine orthologs. To this end, seven complete canine open reading frames were cloned in this family, four of which are novel for the dog, their sequenceswere analyzed, and their functional interactionswere studied in yeasts.Wefound a high degree of overall and domain sequence homology between canine and human proteins. It was slightly higher than between murine and human proteins. Functional interactions between canine pro-apoptotic Bax and Bak and antiapoptotic Bcl-xL, Bcl-w, and Mcl-1 were recapitulated in yeasts. Our data provide support for the notion that systemsbased on canine-derived proteinsmight faithfully reproduce Bcl-2 family member interactions known from other species and establish the yeast as a useful tool for functional studies with canine proteins.

Abstract

Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy.We have assessed the degree of conservation of a set of canine Bcl-2 familymemberswith the human andmurine orthologs. To this end, seven complete canine open reading frames were cloned in this family, four of which are novel for the dog, their sequenceswere analyzed, and their functional interactionswere studied in yeasts.Wefound a high degree of overall and domain sequence homology between canine and human proteins. It was slightly higher than between murine and human proteins. Functional interactions between canine pro-apoptotic Bax and Bak and antiapoptotic Bcl-xL, Bcl-w, and Mcl-1 were recapitulated in yeasts. Our data provide support for the notion that systemsbased on canine-derived proteinsmight faithfully reproduce Bcl-2 family member interactions known from other species and establish the yeast as a useful tool for functional studies with canine proteins.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pathology
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Bcl-2 family, Canine, Cloning, Comparative sequence analysis, Functional yeast study
Language:English
Date:2016
Deposited On:22 Feb 2016 19:20
Last Modified:04 Dec 2016 01:00
Publisher:Elsevier
ISSN:0034-5288
Publisher DOI:https://doi.org/10.1016/j.rvsc.2015.12.001
PubMed ID:26850551

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