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Basal m diurnal variation in mouse perivascular adipose tissue TORC2 activity and expression of its components display


Drägert, Katja; Bhattacharya, Indranil; Hall, Michael N; Humar, Rok; Battegay, Edouard; Haas, Elvira (2016). Basal m diurnal variation in mouse perivascular adipose tissue TORC2 activity and expression of its components display. Biochemical and Biophysical Research Communications, 473(1):317-322.

Abstract

In adipose tissue mTOR complex 2 (mTORC2) contributes to the regulation of glucose/lipid metabolism and inflammatory molecule expression. Both processes display diurnal variations during the course of the day. RICTOR and mSIN1 are unique and essential components of mTORC2, which is activated by growth factors including insulin. To assess whether mTORC2 components display diurnal variations, we analyzed steady state mRNA expression levels of Rictor, mSin1, and mTor in various adipose tissues during a 24 h period. Diurnally regulated expression of Rictor was detected in brown adipose tissues displaying highest mRNA expression levels at the beginning of the 12 h light period (zeitgeber time 2, ZT2). Gene expression patterns of mSin1 and mTor displayed a similar diurnal regulation as Rictor in PVAT while smaller changes were detected for these genes in aorta during the course of the day. Basal mTORC2 activity was measured by phosphorylation of protein kinase C (PKC) α at serine 657 was higher at ZT14 as compared with ZT2 in PVAT. In line, gene expression of inflammatory molecules nitric oxide synthase 2 and tumor necrosis factor α was lower at ZT 14 compared to ZT2. Our findings provide evidence for a diurnal regulation of expression of mTORC2 components and activity. Hence, mTORC2 is possibly an integral part of diurnally regulated signaling pathways in PVAT and possibly in other adipose tissues.

Abstract

In adipose tissue mTOR complex 2 (mTORC2) contributes to the regulation of glucose/lipid metabolism and inflammatory molecule expression. Both processes display diurnal variations during the course of the day. RICTOR and mSIN1 are unique and essential components of mTORC2, which is activated by growth factors including insulin. To assess whether mTORC2 components display diurnal variations, we analyzed steady state mRNA expression levels of Rictor, mSin1, and mTor in various adipose tissues during a 24 h period. Diurnally regulated expression of Rictor was detected in brown adipose tissues displaying highest mRNA expression levels at the beginning of the 12 h light period (zeitgeber time 2, ZT2). Gene expression patterns of mSin1 and mTor displayed a similar diurnal regulation as Rictor in PVAT while smaller changes were detected for these genes in aorta during the course of the day. Basal mTORC2 activity was measured by phosphorylation of protein kinase C (PKC) α at serine 657 was higher at ZT14 as compared with ZT2 in PVAT. In line, gene expression of inflammatory molecules nitric oxide synthase 2 and tumor necrosis factor α was lower at ZT 14 compared to ZT2. Our findings provide evidence for a diurnal regulation of expression of mTORC2 components and activity. Hence, mTORC2 is possibly an integral part of diurnally regulated signaling pathways in PVAT and possibly in other adipose tissues.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine
04 Faculty of Medicine > Center for Integrative Human Physiology
04 Faculty of Medicine > Center of Competence Multimorbidity
Dewey Decimal Classification:610 Medicine & health
Uncontrolled Keywords:Biophysics, Cell Biology, Biochemistry, Molecular Biology
Language:English
Date:22 April 2016
Deposited On:25 May 2016 17:56
Last Modified:19 Aug 2018 03:19
Publisher:Elsevier
ISSN:0006-291X
Funders:Grant No. F-84401-10-01, Grant-No. FK-13-026, FP7/118439
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.bbrc.2016.03.102
PubMed ID:27016480
Project Information:
  • : Funder
  • : Grant ID
  • : Project TitleGrant No. F-84401-10-01
  • : Funder
  • : Grant ID
  • : Project TitleGrant-No. FK-13-026
  • : FunderFP7
  • : Grant ID
  • : Project TitleFP7/118439

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