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Body Fluid Identification Using mRNA Profiling


Roeder, Amy D; Haas, Cordula (2016). Body Fluid Identification Using mRNA Profiling. In: Goodwin, William. Forensic DNA Typing Protocols. New York: Springer, 13-31.

Abstract

RNA analysis is a valuable tool for the identification of the forensically relevant body fluids, saliva, blood, menstrual blood, cervicovaginal fluid, and semen. Multiple human mRNA and bacterial RNA markers have been identified for each of these body fluids. RNA and DNA can be coextracted from the same portion of a sample and RNA markers for different body fluids can be multiplexed in a single PCR, thereby maximizing the number of analyses that can be performed with limited sample material.

Abstract

RNA analysis is a valuable tool for the identification of the forensically relevant body fluids, saliva, blood, menstrual blood, cervicovaginal fluid, and semen. Multiple human mRNA and bacterial RNA markers have been identified for each of these body fluids. RNA and DNA can be coextracted from the same portion of a sample and RNA markers for different body fluids can be multiplexed in a single PCR, thereby maximizing the number of analyses that can be performed with limited sample material.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Legal Medicine
Dewey Decimal Classification:340 Law
610 Medicine & health
Language:English
Date:2016
Deposited On:29 Dec 2016 10:07
Last Modified:02 Feb 2018 11:10
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:1420
ISSN:1064-3745
ISBN:978-1-4939-3595-6
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4939-3597-0_2

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