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Assessment of the occurrence of MCR producing Enterobacteriaceae in Swiss and imported poultry meat


Zurfluh, Katrin; Buess, Smone; Stephan, Roger; Nüesch-Inderbinen, Magdalena (2016). Assessment of the occurrence of MCR producing Enterobacteriaceae in Swiss and imported poultry meat. SDRP Journal of Food Science & Technology, 1(4):online.

Abstract

To determine whether MCR-producing Enterobacteriaceae are present in poultry meat at retail level, 128 fresh meat samples from Switzerland (n=40), Germany (n=69), Hungary (n=6), Denmark (n=6), Austria (n=2), and Italy (n=5), obtained from retail stores were analyzed. The plasmid-mediated colistin resistance gene mcr-1 was detected by PCR in 33 (25.8%) meat samples (5 samples originating from Italy and 28 samples originating from Germany). Thereof, 12 mcr-1 harbouring E. coli were obtained from 12 samples (10 from Germany and two from Italy) by subculturing for further characterization. Despite repeated subculturing, the remaining 21 samples were discarded due to mixed growth with Proteus spp., which possess intrinsic resistance to polymyxins. The isolates belonged to various sequence types (ST) and clonal complexes (CC), including E. coli of the endemic avian and human pathogenic CC10, CC23, and CC155. Further STs included ST156, ST1431 and ST3519, which are associated with humans and animals. Our data highlight the importance of surveillance of retail poultry meat from different countries in context of the global dissemination of MCR-producing E. coli.

Abstract

To determine whether MCR-producing Enterobacteriaceae are present in poultry meat at retail level, 128 fresh meat samples from Switzerland (n=40), Germany (n=69), Hungary (n=6), Denmark (n=6), Austria (n=2), and Italy (n=5), obtained from retail stores were analyzed. The plasmid-mediated colistin resistance gene mcr-1 was detected by PCR in 33 (25.8%) meat samples (5 samples originating from Italy and 28 samples originating from Germany). Thereof, 12 mcr-1 harbouring E. coli were obtained from 12 samples (10 from Germany and two from Italy) by subculturing for further characterization. Despite repeated subculturing, the remaining 21 samples were discarded due to mixed growth with Proteus spp., which possess intrinsic resistance to polymyxins. The isolates belonged to various sequence types (ST) and clonal complexes (CC), including E. coli of the endemic avian and human pathogenic CC10, CC23, and CC155. Further STs included ST156, ST1431 and ST3519, which are associated with humans and animals. Our data highlight the importance of surveillance of retail poultry meat from different countries in context of the global dissemination of MCR-producing E. coli.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2016
Deposited On:23 Jan 2017 09:37
Last Modified:09 Mar 2017 09:37
Publisher:Stift Desk
ISSN:2473-6419
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.15436/JFST.1.4.5

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