Beef production is an important economic activity. In Southern Europe there are two types of beef production systems based on growth paths: continuous (CG) versus discontinuous growth (DG). DG is a traditional system dependent on pasture; whereas in CG animals are supplemented on concentrate feed. We compare the protein abundance profiles of the longissimus thoracis (LT) muscle in CG and DG animals using label-free quantitative proteomics. Twenty three Alentejana male calves (9months-old, 239kg live-weight) were allocated to two feeding regimens. In CG (n=12) production system, animals were fed ad libitum on concentrates plus hay and slaughtered at 18months. In DG (n=11) production system, animals were fed ad libitum on hay from 9 to 15months of age and then the same diet provided to the CG group and slaughtered at 24months. The LT muscle was sampled and protein abundance profiles determined using label-free quantification. We identified 510 proteins, of which 26 showed differential abundance. Several proteins (e.g. Myozenin-2, glyoclythic enzymes and 14-3-3 protein zeta/delta) are proposed as indicators of a more intensive growth path. Myosin binding protein H had higher abundance in the DG group, suggesting it could be associated to discontinuous growth path.