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Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery


Lundstrom, K; Schweitzer, C; Rotmann, D; Hermann, D; Schneider, E M; Ehrengruber, M U (2001). Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery. FEBS letters, 504(3):99-103.

Abstract

Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Adaptation of the SFV technology for mammalian suspension cultures has allowed the production of hundreds of milligrams of recombinant receptor for purification and structural studies. The same SFV stock solutions used for the infection of mammalian cells in culture have also been successfully applied for efficient transgene expression in organotypic hippocampal slices, as well as in vivo in rodent brain.

Abstract

Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Adaptation of the SFV technology for mammalian suspension cultures has allowed the production of hundreds of milligrams of recombinant receptor for purification and structural studies. The same SFV stock solutions used for the infection of mammalian cells in culture have also been successfully applied for efficient transgene expression in organotypic hippocampal slices, as well as in vivo in rodent brain.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:31 August 2001
Deposited On:18 Jan 2017 15:56
Last Modified:22 Jan 2017 06:29
Publisher:Elsevier
ISSN:0014-5793
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/S0014-5793(01)02707-7
PubMed ID:11532440

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