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A disassembly strategy for imaging endogenous pyrophosphate in mitochondria by using an feiii-salen complex


Kumari, Namita; Huang, Huaiyi; Chao, Hui; Gasser, Gilles; Zelder, Felix (2016). A disassembly strategy for imaging endogenous pyrophosphate in mitochondria by using an feiii-salen complex. Chembiochem, 17(13):1211-1215.

Abstract

Inorganic pyrophosphate (PPi) is produced from nucleoside triphosphates in important biosynthetic reactions and is considered a diagnostic marker for various diseases, such as cancer, crystal deposition disease, and arthritis. Traditional methods for biological PPi detection rely on off-line analytics after sample destruction. Molecular probes for imaging this biologically important analyte with temporal and spatial control in living cells are currently in demand. Herein, we report an FeIII–salen complex as the first small reaction-based probe for endogenous mitochondrial PPi following a disassembly approach. Significantly, we successfully applied this complex for the detection of increased cellular PPi levels, and its performance was not affected by the presence of mitochondrial ATP in living cells.

Abstract

Inorganic pyrophosphate (PPi) is produced from nucleoside triphosphates in important biosynthetic reactions and is considered a diagnostic marker for various diseases, such as cancer, crystal deposition disease, and arthritis. Traditional methods for biological PPi detection rely on off-line analytics after sample destruction. Molecular probes for imaging this biologically important analyte with temporal and spatial control in living cells are currently in demand. Herein, we report an FeIII–salen complex as the first small reaction-based probe for endogenous mitochondrial PPi following a disassembly approach. Significantly, we successfully applied this complex for the detection of increased cellular PPi levels, and its performance was not affected by the presence of mitochondrial ATP in living cells.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Uncontrolled Keywords:Organic Chemistry, Molecular Medicine, Biochemistry, Molecular Biology
Language:English
Date:2016
Deposited On:30 Jan 2017 11:26
Last Modified:19 Aug 2018 07:24
Publisher:Wiley-VCH Verlag
ISSN:1439-4227
Funders:University of Zurich Forschungskredit, National Science Foundation of China, Program for Changjiang Scholars and Innovative Research Team at the University of China, Swiss National Science Foundation, University of Zurich Stiftung für wissenschaftliche Forschung
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/cbic.201600195
PubMed ID:27124688
Project Information:
  • : Funder
  • : Grant ID
  • : Project TitleUniversity of Zurich Forschungskredit
  • : Funder
  • : Grant ID
  • : Project TitleNational Science Foundation of China
  • : Funder
  • : Grant ID
  • : Project TitleProgram for Changjiang Scholars and Innovative Research Team at the University of China
  • : FunderSNSF
  • : Grant ID
  • : Project TitleSwiss National Science Foundation
  • : Funder
  • : Grant ID
  • : Project TitleUniversity of Zurich Stiftung für wissenschaftliche Forschung

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