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Evaluation of an in vitro system to simulate equine foregut digestion and the influence of acidity on protein and fructan degradation in the horse´s stomach


Strauch, Saskia. Evaluation of an in vitro system to simulate equine foregut digestion and the influence of acidity on protein and fructan degradation in the horse´s stomach. 2016, University of Zurich, Vetsuisse Faculty.

Abstract

The aim of the present study was to improve an in vitro system in order to collect data on the digestion of different forages in the horse´s upper gastro-intestinal tract. Therefore, in vitro foregut digestion of several forages (grass mixture for horses, grass mixture for cows (GMC), tall fescue, English perennial ryegrass (ER), white clover, lucerne) was performed in two phases with pepsin and amylase, whereas microbial fermentation was neglected. The results are consistent with current data from in vivo studies, including a degradation of crude protein and monosaccharides as well as a relative increase of fibres. Interestingly, a loss of fructan was measured in two feedstuffs (ER/GMC). As acid hydrolysis was suspected to be responsible for this, the effect of different pH-values (2, 3 and 4) on the fructan degradation of ER and GMC was tested subsequently. As expected, the highest degradation of protein was shown at the lowest pH (protein in ER/GMC at pH 2: 6.1/8.2 % DM and at pH 4: 7.7/10.6 % DM), whereas fructan degradation was highest at pH 4 (fructan in ER/GMC at pH 2: 1.63/1.95 % DM and at pH 4: 1.31/0.91 % DM). We presume that not only acidic hydrolysis but also plant enzymes cause the loss of fructans in an acidic environment.

Abstract

The aim of the present study was to improve an in vitro system in order to collect data on the digestion of different forages in the horse´s upper gastro-intestinal tract. Therefore, in vitro foregut digestion of several forages (grass mixture for horses, grass mixture for cows (GMC), tall fescue, English perennial ryegrass (ER), white clover, lucerne) was performed in two phases with pepsin and amylase, whereas microbial fermentation was neglected. The results are consistent with current data from in vivo studies, including a degradation of crude protein and monosaccharides as well as a relative increase of fibres. Interestingly, a loss of fructan was measured in two feedstuffs (ER/GMC). As acid hydrolysis was suspected to be responsible for this, the effect of different pH-values (2, 3 and 4) on the fructan degradation of ER and GMC was tested subsequently. As expected, the highest degradation of protein was shown at the lowest pH (protein in ER/GMC at pH 2: 6.1/8.2 % DM and at pH 4: 7.7/10.6 % DM), whereas fructan degradation was highest at pH 4 (fructan in ER/GMC at pH 2: 1.63/1.95 % DM and at pH 4: 1.31/0.91 % DM). We presume that not only acidic hydrolysis but also plant enzymes cause the loss of fructans in an acidic environment.

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Additional indexing

Item Type:Dissertation
Referees:Liesegang Annette
Communities & Collections:05 Vetsuisse Faculty > Institute of Animal Nutrition
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:2016
Deposited On:02 Feb 2017 12:45
Last Modified:02 Feb 2018 11:57
Number of Pages:16
OA Status:Closed
Related URLs:https://www.recherche-portal.ch/ZAD:default_scope:ebi01_prod010807969 (Library Catalogue)

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