Header

UZH-Logo

Maintenance Infos

7-Rhamnosylated flavonols modulate homeostasis of the plant hormone auxin and affect plant development


Kuhn, Benjamin M; Errafi, Sanae; Bucher, Rahel; Dobrev, Petre; Geisler, Markus; Bigler, Laurent; Zažímalová, Eva; Ringli, Christoph (2016). 7-Rhamnosylated flavonols modulate homeostasis of the plant hormone auxin and affect plant development. Journal of Biological Chemistry, 291(10):5385-5395.

Abstract

Flavonols are a group of secondary metabolites that affect diverse cellular processes. They are considered putative negative regulators of the transport of the phytohormone auxin, by which they influence auxin distribution and concomitantly take part in the control of plant organ development. Flavonols are accumulating in a large number of glycosidic forms. Whether these have distinct functions and diverse cellular targets is not well understood. The rol1-2 mutant of Arabidopsis thaliana is characterized by a modified flavonol glycosylation profile that is inducing changes in auxin transport and growth defects in shoot tissues. To determine whether specific flavonol glycosides are responsible for these phenotypes, a suppressor screen was performed on the rol1-2 mutant, resulting in the identification of an allelic series of UGT89C1, a gene encoding a flavonol 7-O-rhamnosyltransferase. A detailed analysis revealed that interfering with flavonol rhamnosylation increases the concentration of auxin precursors and auxin metabolites, whereas auxin transport is not affected. This finding provides an additional level of complexity to the possible ways by which flavonols influence auxin distribution and suggests that flavonol glycosides play an important role in regulating plant development.

Abstract

Flavonols are a group of secondary metabolites that affect diverse cellular processes. They are considered putative negative regulators of the transport of the phytohormone auxin, by which they influence auxin distribution and concomitantly take part in the control of plant organ development. Flavonols are accumulating in a large number of glycosidic forms. Whether these have distinct functions and diverse cellular targets is not well understood. The rol1-2 mutant of Arabidopsis thaliana is characterized by a modified flavonol glycosylation profile that is inducing changes in auxin transport and growth defects in shoot tissues. To determine whether specific flavonol glycosides are responsible for these phenotypes, a suppressor screen was performed on the rol1-2 mutant, resulting in the identification of an allelic series of UGT89C1, a gene encoding a flavonol 7-O-rhamnosyltransferase. A detailed analysis revealed that interfering with flavonol rhamnosylation increases the concentration of auxin precursors and auxin metabolites, whereas auxin transport is not affected. This finding provides an additional level of complexity to the possible ways by which flavonols influence auxin distribution and suggests that flavonol glycosides play an important role in regulating plant development.

Statistics

Citations

7 citations in Web of Science®
6 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

55 downloads since deposited on 06 Feb 2017
55 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:540 Chemistry
Language:English
Date:4 March 2016
Deposited On:06 Feb 2017 15:26
Last Modified:04 Aug 2017 11:33
Publisher:American Society for Biochemistry and Molecular Biology
ISSN:0021-9258
Funders:Swiss National Science Foundation, Forschungskredit of the University of Zurich, Czech Science Foundation, Novartis foundation
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1074/jbc.M115.701565
PubMed ID:26742840

Download

Download PDF  '7-Rhamnosylated flavonols modulate homeostasis of the plant hormone auxin and affect plant development'.
Preview
Content: Published Version
Filetype: PDF
Size: 2MB
View at publisher