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Assessment of immune responses to fungal infections: identification and characterization of immune cells in the infected tissue


Sparber, Florian; LeibundGut-Landmann, Salomé (2017). Assessment of immune responses to fungal infections: identification and characterization of immune cells in the infected tissue. In: Lion, Thomas. Human Fungal Pathogen Identification : Methods and Protocols. New York: Springer, 167-182.

Abstract

The immune system is important to protect the host from fungal infections. Diverse cell types belonging to the innate or adaptive branch of the immune system act in a tightly coordinated and tissue-specific manner. Experimental mouse models of fungal infections have proved essential for assessing the protective principles against different fungal pathogens. Besides pathological, histological, biochemical and molecular parameters, the analysis of phenotypic and functional aspects of immune cells in infected tissues is key for understanding the mechanisms of antifungal defense. In this chapter, we describe a method based on flow cytometry to assess innate and adaptive immune cells isolated from an in vivo context in a qualitative and quantitative manner.

Abstract

The immune system is important to protect the host from fungal infections. Diverse cell types belonging to the innate or adaptive branch of the immune system act in a tightly coordinated and tissue-specific manner. Experimental mouse models of fungal infections have proved essential for assessing the protective principles against different fungal pathogens. Besides pathological, histological, biochemical and molecular parameters, the analysis of phenotypic and functional aspects of immune cells in infected tissues is key for understanding the mechanisms of antifungal defense. In this chapter, we describe a method based on flow cytometry to assess innate and adaptive immune cells isolated from an in vivo context in a qualitative and quantitative manner.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Candida albicans, Flow cytometry, Host defense, Innate and adaptive immunity, Mouse models, Mucosal tissue
Language:English
Date:2017
Deposited On:23 Feb 2017 12:56
Last Modified:19 Feb 2018 07:38
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:1508
ISSN:1064-3745
ISBN:978-1-4939-6513-7
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4939-6515-1_8
Related URLs:https://www.recherche-portal.ch/ZAD:default_scope:ebi01_prod010808979 (Library Catalogue)
PubMed ID:27837503

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