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Membrane Bound Enzyme Hosted in Liquid Crystalline Cubic Phase for Sensing and Fuel Cells


Nazaruk, Ewa; Landau, Ehud M; Bilewicz, Renata (2014). Membrane Bound Enzyme Hosted in Liquid Crystalline Cubic Phase for Sensing and Fuel Cells. Electrochimica Acta, 140:96-100.

Abstract

Monoolein or phytantriol liquid crystalline cubic phase and carbon nanotubes were employed as the matrices for the immobilization of a membrane bound enzyme–fructose dehydrogenase (FDH). Entrapment within the cubic phase protects the guest protein from chemical and physical degradation, thereby facilitating retention of its native conformation and bioactivity. The mesophase environment was therefore found appropriate for retaining FDH close to the electrode surface. Phytantriol was used as the cubic phase component in case of measurements carried out in biological fluids containing hydrolyzing enzymes. Fructose dehydrogenase shows direct electron transfer when embedded in the matrix, thus does not require any mediators. The bioanode was employed as a biosensing device for fructose. Due to the resistance of fructose dehydrogenase to oxygen it was found useful for the construction of a membrane less biofuel cell. The enzymatic fuel cell based on FDH in the cubic phase film at the anode and laccase at the biocathode showed open circuit potential of 703 ± 10 mV in the presence of 40 mM fructose in buffer solution, and power density of 850 μW cm−2 at 250 mV in solutions under conditions of continuous flow of dioxygen.

Abstract

Monoolein or phytantriol liquid crystalline cubic phase and carbon nanotubes were employed as the matrices for the immobilization of a membrane bound enzyme–fructose dehydrogenase (FDH). Entrapment within the cubic phase protects the guest protein from chemical and physical degradation, thereby facilitating retention of its native conformation and bioactivity. The mesophase environment was therefore found appropriate for retaining FDH close to the electrode surface. Phytantriol was used as the cubic phase component in case of measurements carried out in biological fluids containing hydrolyzing enzymes. Fructose dehydrogenase shows direct electron transfer when embedded in the matrix, thus does not require any mediators. The bioanode was employed as a biosensing device for fructose. Due to the resistance of fructose dehydrogenase to oxygen it was found useful for the construction of a membrane less biofuel cell. The enzymatic fuel cell based on FDH in the cubic phase film at the anode and laccase at the biocathode showed open circuit potential of 703 ± 10 mV in the presence of 40 mM fructose in buffer solution, and power density of 850 μW cm−2 at 250 mV in solutions under conditions of continuous flow of dioxygen.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:2014
Deposited On:09 Feb 2017 14:24
Last Modified:09 Feb 2017 14:24
Publisher:Elsevier
ISSN:0013-4686
Funders:Grant PSPB 079/2010 to RB and EL from Switzerland through the Swiss Contribution to the enlarged European Union.
Publisher DOI:https://doi.org/10.1016/j.electacta.2014.05.130

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