Header

UZH-Logo

Maintenance Infos

High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)


Laroucau, K; Thierry, S; Vorimore, F; Blanco, K; Kaleta, E; Hoop, R; Magnino, S; Vanrompay, D; Sachse, K; Myers, G S A; Bavoil, P M; Pourcel, C; Vergnaud, G (2008). High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA). Infection, Genetics and Evolution, 8(2):171-181.

Abstract

A multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9 avian reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical origins. Comparative analysis of the MLVA results and those obtained from currently available methods including serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of discrimination, with 20 distinct patterns identified to date. The newly developed MLVA system therefore provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different origins that is suitable for molecular epidemiological studies.

Abstract

A multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9 avian reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical origins. Comparative analysis of the MLVA results and those obtained from currently available methods including serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of discrimination, with 20 distinct patterns identified to date. The newly developed MLVA system therefore provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different origins that is suitable for molecular epidemiological studies.

Statistics

Citations

30 citations in Web of Science®
28 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

129 downloads since deposited on 10 Mar 2009
17 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Bacteriology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:March 2008
Deposited On:10 Mar 2009 20:50
Last Modified:05 Apr 2016 13:00
Publisher:Elsevier
ISSN:1567-1348
Publisher DOI:https://doi.org/10.1016/j.meegid.2007.12.002
PubMed ID:18242149

Download

Preview Icon on Download
Preview
Content: Accepted Version
Filetype: PDF
Size: 1MB
View at publisher
Preview Icon on Download
Filetype: PDF - Registered users only
Size: 1MB