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Rapid microfluidic double-jump mixing device for single-molecule spectroscopy


Dingfelder, Fabian; Wunderlich, Bengt; Benke, Stephan; Zosel, Franziska; Zijlstra, Niels; Nettels, Daniel; Schuler, Benjamin (2017). Rapid microfluidic double-jump mixing device for single-molecule spectroscopy. Journal of the American Chemical Society, 139(17):6062-6065.

Abstract

We introduce a microfluidic double-jump mixing device for investigating rapid biomolecular kinetics with confocal single-molecule spectroscopy. This device enables nonequilibrium dynamics to be probed, e.g., transiently populated intermediates that are inaccessible with existing single-molecule approaches. We demonstrate the potential and reliability of the method on time scales from milliseconds to minutes by investigating the coupled folding and binding reaction of two intrinsically disordered proteins and the conformational changes occurring in a large cytolytic pore-forming toxin.

Abstract

We introduce a microfluidic double-jump mixing device for investigating rapid biomolecular kinetics with confocal single-molecule spectroscopy. This device enables nonequilibrium dynamics to be probed, e.g., transiently populated intermediates that are inaccessible with existing single-molecule approaches. We demonstrate the potential and reliability of the method on time scales from milliseconds to minutes by investigating the coupled folding and binding reaction of two intrinsically disordered proteins and the conformational changes occurring in a large cytolytic pore-forming toxin.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:3 May 2017
Deposited On:07 Aug 2017 10:06
Last Modified:10 Apr 2018 00:00
Publisher:American Chemical Society (ACS)
ISSN:0002-7863
Additional Information:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher.
OA Status:Closed
Publisher DOI:https://doi.org/10.1021/jacs.7b02357
PubMed ID:28394601

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