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Identification of fungal T cell epitopes by mass spectrometry-based proteomics


Roschitzki, Bernd; LeibundGut-Landmann, Salomé (2017). Identification of fungal T cell epitopes by mass spectrometry-based proteomics. Methods in Molecular Biology, 1625:65-74.

Abstract

CD4(+) T cells play a key role in host defense against many fungal infections. T cells are also implicated in vaccine immunity to fungi. To date, only a small number of fungal antigens have been identified. Knowing the antigenic determinants of fungi-specific T cells greatly facilitates the detection, enumeration and characterizes the antifungal T cells and it constitutes an important step toward the design and development of vaccination strategies. This chapter describes a method of MHC-II ligand elution and mass spectrometric analysis to identify naturally processed and presented fungal peptide epitopes.

Abstract

CD4(+) T cells play a key role in host defense against many fungal infections. T cells are also implicated in vaccine immunity to fungi. To date, only a small number of fungal antigens have been identified. Knowing the antigenic determinants of fungi-specific T cells greatly facilitates the detection, enumeration and characterizes the antifungal T cells and it constitutes an important step toward the design and development of vaccination strategies. This chapter describes a method of MHC-II ligand elution and mass spectrometric analysis to identify naturally processed and presented fungal peptide epitopes.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Fungal antigens; MHC-II ligand elution; Mass spectrometry; Naturally processed and presented T cell epitopes; T helper cells
Language:English
Date:2017
Deposited On:23 Aug 2017 17:11
Last Modified:23 Aug 2017 17:11
Publisher:Springer
ISSN:1064-3745
Publisher DOI:https://doi.org/10.1007/978-1-4939-7104-6_5
PubMed ID:28584983

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