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Horizontal and vertical distribution of CD3+ lymphocytes in the intestine of healthy adult and neonatal dogs


Pfammatter, Nadia; Luckschander, N; Jakob, S; Hartnack, S; Brunner, T; Burgener, Iwan A (2017). Horizontal and vertical distribution of CD3+ lymphocytes in the intestine of healthy adult and neonatal dogs. Journal of Clinical Gastroenterology and Hepatology, 1(3):21.

Abstract

T-lymphocytes are considered to play an essential role in the pathogenesis of inflammatory bowel disease. The goal of this study was an objective determination of the distribution of gastrointestinal CD3+ lymphocytes in a standardized dog population as a gold standard for further investigations. Full thickness biopsies were obtained from seven different localizations from stomach to colon from six adults and four neonatal healthy Beagle dogs. The tissue was stained with an anti-CD3 antibody. The positive cells were counted in an area of 200,000 μm2 at four different sites per localization. In adult dogs, the horizontal distribution showed a maximum of CD3+ lymphocytes in the duodenum and jejunum. In the stomach, almost no positive cells were observed. The vertical distribution revealed an accumulation in the villi. The neonatal dogs showed a similar distribution pattern, but on average ten times less CD3+ lymphocytes in the analogous localizations and smaller differences between localizations.

Abstract

T-lymphocytes are considered to play an essential role in the pathogenesis of inflammatory bowel disease. The goal of this study was an objective determination of the distribution of gastrointestinal CD3+ lymphocytes in a standardized dog population as a gold standard for further investigations. Full thickness biopsies were obtained from seven different localizations from stomach to colon from six adults and four neonatal healthy Beagle dogs. The tissue was stained with an anti-CD3 antibody. The positive cells were counted in an area of 200,000 μm2 at four different sites per localization. In adult dogs, the horizontal distribution showed a maximum of CD3+ lymphocytes in the duodenum and jejunum. In the stomach, almost no positive cells were observed. The vertical distribution revealed an accumulation in the villi. The neonatal dogs showed a similar distribution pattern, but on average ten times less CD3+ lymphocytes in the analogous localizations and smaller differences between localizations.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Uncontrolled Keywords:Beagle; T lymphocytes; Inflammatory bowel disease; Lymphocytic-plasmacytic enteritis; CD3; Canines.
Language:English
Date:24 August 2017
Deposited On:18 Sep 2017 13:56
Last Modified:18 Sep 2017 13:56
Publisher:Insight Medical Publishing
Free access at:Official URL. An embargo period may apply.
Official URL:http://www.imedpub.com/articles/horizontal-and-vertical-distribution-of-cd3-lymphocytes-in-the-intestine-ofhealthy-adult-and-neonatal-dogs.php?aid=20066

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